高效液相色谱-质谱联用检测水产品中氧化三甲胺

Determination of trimethylamine-N-oxide in aquatic product using high per-formance liquid chromatography-tandem mass spectrometry

中文关键词:  氧化三甲胺  高效液相色谱-质谱联用法  水产品

英文关键词:trimethylamine-N-oxide  high performance liquid chromatography-tandem mass spectrometry  aquatic product

中文摘要:

      目的 建立水产品中氧化三甲胺(trimethylamine-N-oxide, TMAO)的高效液相色谱法-质谱联用(HPLC-MS/MS)分析方法, 并对2种常见水产品的TMAO含量进行测定。方法 待测物通过色谱柱分离, 以10 mmol/L甲酸铵为流动相A, 含5 mmol/L甲酸铵、25%甲醇、0.1%甲酸溶液为流动相B进行梯度洗脱, 流速为0.8 mL/min, 采用多反应监测(MRM)检测不同撞击电压下母离子碎裂情况, 并选择合适电压下的离子对定量; 利用所建立方法测定阿根廷鱿鱼和凡纳宾对虾中TMAO含量。结果 当撞击电压均设定为20 eV, 确定定量离子对为m/z 76→58.2时, 5~1000 μg/L范围内线性关系良好; 4种水产品TMAO回收率在83.15%~90.23%之间。结论 所建HPLC-MS/MS测定TMAO的方法, 特异性好, 灵敏度高, 快速准确, 可用于水产品中TMAO含量的分析测定。

英文摘要:

      Objective To develop a method for measurement of trimethylamine-N-oxide (TMAO) in aquatic products by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and detect the TMAO content in 2 kinds of familiar aquatic products. Methods The analytes were separated on a chromatographic column at a flow rate of 0.8 ml/min using 10 mmol/L ammonium as mobile phase A and the solution contained 5 mmol/L ammonium formate, 25% methanol, 0.1% formic as mobile phase B. Multiple-reaction monitoring (MRM) was used to determine the transition of precursor ion to product ion at a series collision energy and one ion pair at the appropriate collision energy was selected to quantification. The TMAO content in Illex argentinuscastellanos and Litopenaeus vannamei was determined using the developed method. Results The method had a good linear relationship ranged 5~1000 μg/L when the collision energy was set to 20 eV and m/z 76→58.2 was selected as quantitative ion pair as well. The recovery rates of 4 kinds of aquatic products were 83.15%~90.23%. Conclusion The established HPLC-MS/MS method for TMAO showed good specificity, sensitivity, and simplicity, which was applicable for monitoring TMAO in aquatic products.