高效液相色谱法测定乳与乳制品中 L-羟脯氨酸的含量

Detection of L-hydroxyproline in the milk and dairy products by high per-formance liquid chromatography

中文关键词:  L-羟脯氨酸  乳与乳制品  高效液相色谱法

英文关键词:L-hydroxyproline  milk and dairy products  high performance liquid chromatography

中文摘要:

      目的 建立高效液相色谱法测定乳与乳制品中L-羟脯氨酸的含量。方法 样品水解衍生后采用C18 色谱柱, 以乙腈和10 mmol/L乙酸钠(pH=5.0)为流动相, 梯度洗脱, 流速为1.0 mL/min, 柱温35 ℃, 荧光检测器进行检测。结果 L-羟脯氨酸检出限为0.5 mg/kg, 线性范围为1.0~100.0 μg/mL, 加标回收率在95.3%~102.7%之间, 相对标准偏差在1.46%~7.56%之间。结论 该方法操作简便、准确、快速, 提高了样品检出灵敏度, 适用于测定乳与乳制品中L-羟脯氨酸的含量。

英文摘要:

      Objective To establish a method for the detection of L-hydroxyproline in milk and dairy products by high performance liquid chromatography. Methods L-hydroxyproline in samples was extracted by derivation and separated on a C18 column using acetonitrile and 10 mmol/L sodium acetate(pH=5.0)as the mobile phase at a flow rate of 1.0 mL/min with the column temperature of 35 ℃, and then determined by fluo-rescence detector. Results The detection limit of L-hydroxyproline was 0.5 mg/kg. There was a good linearity in the range of 1.0~100.0 μg/mL, the recovery of standard addition was 95.3%~102.7%, and the relative standard deviation was 1.46%~7.56%. Conclusion This method is simple, accurate, rapid, and can improve the detection sensitivity, which is suitable for the determination of L-hydroxyproline in milk and dairy products.