响应面法优化沙棘籽渣水溶性蛋白质提取工艺研究及其氨基酸组成分析

Optimization of extraction processing for soluble protein of seed residue of sea buckthorn using the response surface analysis and amino acid composition analysis

中文关键词:  响应面分析  沙棘籽渣  水溶性蛋白质  氨基酸组成分析

英文关键词:sea buckthorn  response surface methodology  water-soluble protein  composition of amino acid

中文摘要:

      目的 利用响应面法优化沙棘籽渣水溶性蛋白质的提取工艺条件, 测定沙棘籽渣水溶性蛋白质的氨基酸组成。方法 在单因素试验的基础上, 以水溶性蛋白质提取率为试验指标, 用响应面法研究液料比、提取温度、pH、提取时间及其交互作用对沙棘籽渣水溶性蛋白质提取率的影响。对所得蛋白质进行氨基酸组成分析。结果 响应面法优化沙棘籽渣水溶性蛋白质最佳提取工艺条件为: 提取时间88 min、提取温度60 ℃、pH 11、液料比15:1 mL/g。各因素对蛋白质提取作用的大小依次为: 提取时间>提取温度>pH>液料比。利用响应面法得到的二次多项式回归方程数学模型, 沙棘籽渣水溶性蛋白提取率Y=38.29+0.44A?0.33B+3.49C+0.63D? 0.56A2?0.97B2+1.09C2?0.39D2+0.0025AB?0.0025AC+8.414×10?3AD+8.414×10?3BC+8.414×10?3BD?0.01CD, 能较准确地预测试验结果。所得沙棘籽渣水溶性蛋白质由17种氨基酸组成。结论 本研究结果可为沙棘籽渣水溶性蛋白质的提取和应用提供理论依据。

英文摘要:

      Objective To optimize the extraction processing of water-soluble protein in sea buckthorn seed by response surface method and analyze the amino acid composition of the water-soluble protein. Methods The effects of the ratio of liquid, extraction temperature, pH and extraction time and their interactions on the protein yield were researched based on the single-factor test and the amino acid composition of the water-soluble protein was analyzed. Results The optimum extraction conditions were as follows: extraction time of 88 min, extraction temperature of 60 ℃, pH 11, liquid-to-solid ratio 15:1 mL/g. The influence order of factors to protein extracting was: extraction time>extraction temperature>pH>liquid-to-solid ratio. The mathematic model of the quadratic polynomial regression equation was Y=38.29+0.44A?0.33B+ 3.49C+0.63D?0.56A2?0.97B2+1.09C2?0.39D2+0.0025AB?0.0025AC+8.414×10?3AD+8.414×10?3BC+8.414×10?3BD? 0.01CD, which was obtained through the response surface method and could exactly predict the yield of protein. Water-soluble protein was made up of 17 kinds of amino acids. Conclusion This study will provide theoretical basis for the extraction and the application of the water-soluble protein of seed residue of sea buckthorn.