液相色谱-质谱联用法测定保健食品中 9种脂溶性维生素

Determination of 9 kinds of fat-soluble vitamins in healthy foods by liquid chromatography-mass spectrometry

中文关键词:  保健食品  脂溶性维生素  液相色谱质谱联用法

英文关键词:healthy food  fat-soluble vitamin  liquid chromatography-mass spectrometry

中文摘要:

      目的 建立同时测定保健食品中9种脂溶性维生素的液相色谱-质谱联用法。方法 用适合的有机溶剂充分溶解试样中的脂溶性维生素, 超声提取一段时间后, 经液相色谱-质谱联用仪进行测定。采用标准样品定量离子外标法定量, 以ACQUITY UPLC BEH C18色谱柱, 对色谱条件、质谱条件进行优化后, 通过分子离子峰、二级碎片、色谱保留时间等信息, 对片剂、软胶囊基质中的9种脂溶性维生素进行定量测定。结果 9种脂溶性维生素标准品溶液浓度和检测响应的线性关系良好, 其相关系数r均大于0.99; 检出限为0.226~23.5 μg/L, 定量限为1.79~70.6 μg/L, 方法准确度、精密度、重复性均可满足要求。结论 运用液相色谱质谱联用法可同时测定保健食品中的9种脂溶性维生素, 并在很大程度上简化了提取过程、提高了实验效率, 该方法快捷、简便、准确。

英文摘要:

      Objective To establish a method for the simultaneous determination of fat-soluble vitamins in healthy foods by liquid chromatography-mass spectrometry (LC-MS). Methods The samples were extracted by ultrasonic in organic solvents, then determined by LC-MS. Quantitative ions of standards in external standard method were used as analysis of quantification. The chromatography and mass spectrometry was optimized using an ACQUITY UPLC BEH C18 column. Nine kinds of fat-soluble vitamins added in health foods were determined qualitatively and quantitatively based on the information of molecular ion mass, speci?c fragment ions and chromatographic retention. Results The linearity of 9 kinds of fat-soluble vitamins was good with r>0.99. Limits of determination of 9 kinds of vitamins were 0.226~23.5 μg/L, and limits of quantitation were 1.79~70.6 μg/L. Precision, accuracy and repeatability was all satisfied. Conclusion This method is simple and reproducible, which can be applied in analysis of fat-soluble vitamins in healthy foods.