液相色谱/四级杆飞行时间质谱分析烟酸芽胞 杆菌发酵产物苯乳酸

Analysis on phenyllactic acid from Bacillus niacini based on liquid chroma-tography-hybrid quadrupole time-of-flight mass spectrometry

中文关键词:  液相四级杆飞行时间质谱: 烟酸芽胞杆菌  胞外代谢物  苯乳酸

英文关键词:liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry  Bacillus niacini  extracellular metabolites  phenyllactic acid

中文摘要:

      目的 对烟酸芽胞杆菌Bacillus niacini FJAT-14202来源的苯乳酸成分进行分析与检测。方法 采用液相色谱/四级杆飞行时间质谱(liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry)分析烟酸芽胞杆菌FJAT-14202发酵液中胞外代谢物的成分。利用MassHunter软件, 对原始数据进行分子特征提取, 通过Metlin代谢物质谱数据库检索比对获得代谢物的信息。结果 在烟酸芽胞杆菌FJAT-14202发酵液中检测到811种代谢物, 通过Metlin谱库搜索获得初步鉴定的有165种。其中, 发现了具有生物活性的物质苯乳酸, 其匹配得分达到96.52, 占发酵液总代谢物相对含量的5.31%, 保留时间分别为2.1057 min, 精确质量数为166.0552。结论 苯乳酸的发现为烟酸芽胞杆菌的开发与利用提供理论依据。

英文摘要:

      Objective To analyze and test on active substance from Bacillus niacini FJAT-14202. Methods Extracellular metabolites of Bacillus niacini FJAT-14202 were analyzed by liquid chromatography- hybrid quadrupole time-of-flight mass spectrometry. Molecular feature extraction (MFE) and database retrieval were applied by Agilent Mass Hunter software, for the information overall metabolites. Results Totally 811 compounds were detected, and 165 of them were identified by library searching of Metlin database. The match score of phenyllactic acid was 96.52 and its relative content was 5.31%. The retention time of phenyllactic acid was about 2.1057 min, and its accurate mass was 166.0552. Conclusion The results provide the theory basis for development and utilization of phenyllactic acid from Bacillus niacini.