快速溶剂萃取仪提取保健食品中人参皂苷 条件优化

Optimization of extraction condition for total ginsenosides from health food by accelerated solvent extraction

中文关键词:  快速溶剂萃取仪  人参总皂苷  提取

英文关键词:accelerated solvent extraction  total ginsenosides  extraction

中文摘要:

      目的 建立快速溶剂萃取仪对保健食品中人参总皂苷进行在线提取的方法。方法 通过正交试验设计, 采用快速溶剂萃取法确定最佳工艺。结果 ASE快速溶剂萃取仪法萃取仪处理样品最佳工艺参数为提取温度140 ℃、提取时间5 min, 循环次数3次, 采用最佳工艺提取所测6种保健食品中人参总皂苷的质量分数为0.24~1.60 g/100 g。在0.005~1.0 mg内线性关系良好(r=0.9998), 加标回收率为92.9%~102.3%, RSD为1.8%~2.5%(n=6)。结论 快速溶剂萃取仪能够准确快速提取检测保健食品中的人参总皂苷, 可以考虑应用到其他保健食品中功效成分的测定中。

英文摘要:

      Objective To establish a method for the extraction of total ginsenosides from health food online by accelerated solvent extraction (ASE). Methods The optimal extraction conditions were defined by orthogonal test using ASE. Results The best process parameters with ASE were as follows: temperature 140 ℃, time 5 min, times 3. The ginsenosides mass fraction of the 6 kinds of health foods measured by the best optimum extraction was 0.24~1.60 g/100 g. There was a good linear relationship (r=0.9998) in the range of 0.005~1.0 mg, the recovery was 92.9%~102.3%, and the RSD was 1.8%~2.5% (n=6). Conclusion Total ginsenosides from health food is extracted quickly and detected accurately by accelerated solvent extraction, which can be used for the measurement of other health food effectiveness ingredients.