新型QuEChERS方法结合液相色谱串联质谱法快速测定猪肝中-受体激动剂残留

Rapid determination of -agonists residuals in pig liver by liquid chromatography- tandem mass spectrometry combined with novel QuEChERS method

中文关键词:  -受体激动剂  残留  QuEChERS  液相色谱串联质谱法

英文关键词:-agonists  residuals  QuEChERS  liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立一种基于新型QuEChERS材料测定猪肝中?-受体激动剂的方法。方法 猪肝样品经酶解、酸化乙腈提取后, 提取液用增强基质去脂质材料(EMR-lipid)脱脂, 盐析浓缩后经液相色谱质谱联用仪测定, 稳定同位素内标法定量。结果 19种?-受体激动剂在0.5~10.0 ?g/kg范围内线性关系良好, 高、中、低浓度日内、日间精密度小于20%, 回收率在60%~120%之间。结论 本方法简便快速, 适宜于大批量样品的快速筛选和定量分析。

英文摘要:

      Objective To develop a method to determine the ?-agonists residuals in pig liver based on a novel QuEChERS material. Methods The sample of pig liver was enzymatic hydrolysis and extracted by aci-dified acetonitrile. The extraction was cleaned-up by enhanced matrix removal (EMR-lipid) material. After polished and salted out, the analytes were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Stable isotopes were used as internal standard. Results Nineteen ?-agonists were detected at the range between 0.5 ?g/kg and 10.0 ?g/kg with good linearity. The intra- and inter-precision were lower than 20%, and the recoveries were between 60% and 120% with high, medium and low concentrations. Conclusions The method is simple, rapid and effective, and it is suitable for rapid screening and quantitative analysis of large scale samples.