高效液相色谱法检测果冻中的姜黄素类化合物

Determination of curcuminoids in jelly by high performance liquid chroma-tography

中文关键词:  高效液相色谱法  果冻  姜黄素类化合物

英文关键词:high performance liquid chromatography  jelly  curcuminoids

中文摘要:

      目的 建立果冻制品中姜黄素类化合物的高效液相色谱测定方法。方法 样品用甲醇水混合溶液(7+3, V:V)提取, 经过0.45 μm微孔滤膜过滤后进样分析。色谱柱: Kinetex PFP五氟苯基柱; 流动相: 0.1%甲酸水和0.1%甲酸乙腈; 洗脱方式: 等度; 检测器波长 420 nm ; 流速为1 mL/min; 柱温: 室温; 进样量为4.0 μL; 采用色谱峰保留时间定性, 外标法峰面积定量。结果 标准溶液在1 μg/mL~100 μg/mL范围内线性良好, 相关系数0.999, 平均回收率在93.0%~103.2%之间, 相对标准偏差2.73%~4.89%。结论 该方法具有样品预处理简单等特点, 实测中发现可吸果冻和凝胶果冻中普遍含有姜黄素成分, 姜黄素类化合物总含量在1~15 mg/kg之间。

英文摘要:

      Objective A sensitive and efficient method based on high performance liquid chromatography (HPLC) was developed for simultaneous identification and quantification of curcuminoids in jelly. Method Jelly samples were diluted and extracted by methanol: water (7:3). Using Kinetex PFP (five fluorinated phenyl) column, with a 0.1% formic acid water solution and 0.1% formic acid acetonitrile solution as mobile phase. The flow rate was 1.0 mL/min and detection wavelength was 420 nm under room temperature with 4.0 μL injection volume. Results The recoveries of curcuminoids were in range of 93.0%~103.2%, the relative standard deviation was 2.73%~4.89%. It showed a good linearity in 1.0~100.0 μg/mL with the correlation coefficients (r2) of 0.999. Conclusion Curcuminoids were detected in gel jelly and straw jelly, and the level of curcuminoids was between 1~15 mg/kg.