超高效液相色谱-串联质谱法检测动物血浆中 克伦特罗、莱克多巴胺、沙丁胺醇

Determination of clenbuterol, ractopamine and salbutamol in animal plasma by ultra high performance liquid chromatography-tandem mass spectrometry

中文关键词:  血浆  克伦特罗  莱克多巴胺  沙丁胺醇  超高效液相色谱-串联质谱法

英文关键词:plasma  clenbuterol  ractopamine  salbutamol  ultra performance liquid chromatography –tandem mass spectrometry

中文摘要:

      目的 建立动物血浆中克伦特罗、莱克多巴胺、沙丁胺醇的超高效液相色谱-串联质谱检测方法。 方法 采用乙酸铵缓冲液酶解提取血浆中的目标化合物, 提取液经混合阳离子交换柱净化, 采用超高效液相色谱-串联质谱(ultra performance liquid chromatography–tandem mass spectrometry)多反应监测模式检测, 内标法定量。结果 克伦特罗、莱克多巴胺、沙丁胺醇回收良好, 线性相关系数均大于0.99, 测定低限均为0.5 μg/kg。结论 该方法适用于动物血浆中克伦特罗、莱克多巴胺、沙丁胺醇残留的检测。

英文摘要:

      Objective A method based on ultra performance liquid chromatography-tandem mass spec-trometry(UPLC-MS/MS) has been developed for the determination of beta-agonists residues including clenbuterol, ractopamine, and salbutamol in animal plasma. Method The analytes were extracted with NH4AC solution from samples and hydrolysised with beta-glucuronidase overnight at 37 ℃. The extract was filtered and the filtrate was cleaned by Oasis MCX solid phase extraction cartridge. Quantification of the 3 analytes was achieved by UPLC-MS/MS with multiple reaction monitoring (MRM) using internal standard method. Results The method had a good recovery for the clenbuterol, ractopamine, and salbutamol, and the correlation coefficient was more than 0.99 with the limit of quantitation (LOQ) 0.5 μg/kg. Conclusion The method is suitable for the determination of clenbuterol, ractopamine, and salbutamol residues in animal plasma.