刺参多糖的提取纯化及其组分的分析

Purification of polysaccharides and analysis of their contents and compositions in sea cucumbers (Apostichopus japonicas)

中文关键词:  刺参  多糖  纯化  组分  分析

英文关键词:sea cucumbers  polysaccharides  purified  component content  analysis

中文摘要:

      目的 研究圈养刺参多糖的组成。方法 以大连圈养刺参为研究对象, 采用酶解醇沉法提取刺参的粗多糖, 并通过DEAE-Sepharose Fast Flow阴离子交换树脂进行进一步纯化, 并分别采用高效液相色谱法和红外光谱法对多糖的单糖组成及其含量和多糖的结构进行分析。结果 提取的刺参粗多糖含量为62.30%, 蛋白质含量为14.27%, 纯度较好, 经纯化后得到的三个组分峰(峰1、峰2和峰3), 除甘露糖外, 组分峰2和组分峰3中各单糖含量间均存在显著性差异(P<0.05), 且组分峰3多糖的质量比最高, 为36.45%; 刺参多糖中的单糖以岩藻糖、氨基半乳糖、葡萄糖醛酸为主, 除葡萄糖外, 组分峰3中的其他单糖含量均高于组分峰2; 组分峰3的结构较复杂。结论 本文为海参多糖的开发与利用提供了技术支撑。

英文摘要:

      Objective To analyze the composition of polysaccharide of farmed sea cucumbers (Apostichopus japonicas). Methods Dalian sea cucumber was used as raw materials. Crude polysaccharides of sea cucumber was extracted by enzyme alcohol sink method and purified by DEAE-Sepharose Fast Flow anion exchange chromatography. The structure and compositions of purified polysaccharide were analyzed by infrared spectral and precolumn derivatization high performance liquid chromatography, respectively. Results The polysaccharides and protein content were 62.30% and 14.27%. Three peaks were obtained after purified by DEAE-Sepharose Fast Flow anion exchange chromatography. There were significant difference between peak 2 and peak 3 (except for mannose). The polysaccharide of peak 3 of mass ration was 36.45%. Fucose, galactosamine and glucuronic acid were the primary monosaccharide in polysaccharides of farmed sea cucumbers from Dalian. Except for glucose, the content of the above 3 kinds of monosaccharide were all higher in peak 3 than that in peak 2. The constitution of peak 3 was complex. Conclusion This article provides technical support for the development and utilization of polysaccharides of sea cucumber.