MS-222对大菱鲆麻醉效果及富集消除规律研究

Anesthesia effects and accumulation and elimination regularity of MS-222 in turbot Scophthalmus maxinus

中文关键词:  3-氨基苯甲酸乙酯甲基磺酸盐  大菱鲆  麻醉  富集  消除

英文关键词:tricaine methane sulphonate  Scophthalmus maxinus  anesthesia  accumulation  elimination

中文摘要:

      目的 确立大菱鲆的有效麻醉浓度, 研究3-氨基苯甲酸乙酯甲基磺酸盐(MS-222)在大菱鲆体内的生物富集和消除规律。方法 考察大菱鲆在不同MS-222浓度下麻醉、苏醒过程中的行为变化以及呼吸频率的变化, 采用液相色谱-串联质谱法测定大菱鲆组织中的药物水平。结果 MS-222麻醉大菱鲆的有效浓度为60 mg/L, 在此浓度下, 鱼体能够在5 min之内达到第IV期麻醉状态, 5 min之内苏醒恢复。大菱鲆肝脏中MS-222的富集浓度高于肌肉, 经48 h消除实验后肌肉和肝脏中的MS-222均低于1 μg/kg, 其消除半衰期分别为13.14 h(肌肉)和14.15 h(肝脏)。结论 在水温不低于(12±0.3) ℃条件下, 建议将MS-222的休药期定为3天。

英文摘要:

      Objective To establish effective anesthesia concentrations and study the regularity of accumulation and elimination of tricaine methanesulfonate (MS-222) in muscle and liver of turbot. Methods Under different concentrations of MS-222 anesthesia, waking up in the process of behavior change and the change of respiratory frequency were studied in turbot. The residues were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Results The effective concentration of MS-222 for turbot was 60 mg/L, and under this condition, it reached stage IV anesthesia and recovered within 5 min. The accumulation amount of turbot liver was higher than that of the muscle. The MS-222 concentration was eliminated below safe quantity (1 μg/kg) in muscle and liver after 48 h, and the elimination half-life were 13.14 h (muscle) and 14.15 h (liver). Conclusion Under the condition of this experiment, it was recommended withdrawal period of 3 d.