高效液相色谱-质谱联用法测定乳品中的左旋肉碱

Determinatin of L-carnitine in milk products by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  婴儿配方粉  乳品  左旋肉碱  高效液相色谱-质谱联用仪

英文关键词:milk products  L-carnitine  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立高效液相色谱串联质谱法测定乳品中左旋肉碱的含量。方法 样品中的左旋肉碱用乙腈水(3:1, V:V)溶液提取, 提取液经碱皂化后使溶液中结合态的左旋肉碱游离出来。提取液经酸液中和后, 高速离心去除杂质, 取上清液并用微孔滤膜过滤, 稀释后用高效液相色谱-质谱联用仪进行测定和确证。结果 待测物质在2~100 ng/mL范围内呈良好的线性关系(r2=0.9941), 左旋肉碱在不同基质中的加标回收率为66.3%~110.5%, 精密度为2.0%~8.8%。结论 此法可满足日常进出口乳品中左旋肉碱含量的测定工作, 同其他方法相比具有准确定性、操作简单、省时高效的特点。

英文摘要:

      Objective To establish a method for the detection of L-carnitine in milk products by high performance liquid chromatography-mass spectrometry (HPLC-MS/MS). Methods L-carnitine in samples was extracted by acetonitrile:water (3:1, V:V) solution. The combined L-carnitine was released by alkaline saponification. After neutralized by acid, the extraction was centrifuged to discard impurity. Then superna-tant was filtered by syringe filter (0.2 μm) and diluted. Finally, the sample was measured by HPLC-MS/MS. Results It showed a good linearity of material in 2~100 ng/mL (r2=0.9941). The recoveries of L-carnitine in different matrix were 66.3%~110.5%, with the precision of 2.0%~8.8%. Conclusion This method can meet the daily inspection work of import and export of L-carnitine in milk products. Compared with other methods, it has the characteristics of exact determination, simple operation, time saving and high efficiency.