猪尿中盐酸克伦特罗测量结果不确定度的评价

Evaluation of the uncertainty in determination of clenbuterol hydrochloride residue in pig urine

中文关键词:  盐酸克伦特罗  液相色谱-质谱法  不确定度  评价

英文关键词:clenbuterol hydrochloride  liquid chromatography-mass spectrometry  uncertainty  evaluation

中文摘要:

      目的 提高液相色谱-质谱法测量猪尿中盐酸克伦特罗的准确度和可靠性。方法 建立液相色谱-质谱法测定猪尿中盐酸克伦特罗结果不确定度评定的数学模型, 综合分析测量过程中的不确定度来源, 同时对结果的不确定度进行分析。结果 液相色谱-质谱法测猪尿中盐酸克伦特罗引入不确定度的主要因素是仪器设备、标准曲线线性拟合和标准曲线系列配置。结论 在实际检测中可通过加强对实验仪器设备的维护保养、控制标准曲线校准过程和标准溶液的配制过程, 降低测量结果的不确定度。

英文摘要:

      Objective To improve the accuracy and reliability in the determination of clenbuterol hydrochloride in pig urine by liquid chromatography-mass spectrometry (LC-MS). Methods The mathematical model was set up to evaluate the uncertainty of LC-MS method. The source of uncertainty and the result were comprehensively analyzed. Results The main factors of the uncertainty were instruments, standard curve linear fitting and standard curve series configuration. Conclusion The maintenance of experimental instruments, the control of preparation of standard curve and the standard solution calibration process can strengthen in actual detection to reduce the uncertainty of measurement results.