分散固相萃取-高效液相色谱法测定水果中三氯生及三氯卡班

Determination of triclosan and triclocarban in fruit by dispersive solid-phase extraction and high performance liquid chromatography

中文关键词:  分散固相萃取  高效液相色谱法  三氯生  三氯卡班

英文关键词:dispersive solid-phase extraction  high performance liquid chromatography  triclosan  triclocarban.

中文摘要:

      目的 建立分散固相萃取-高效液相色谱法测定水果中三氯生及三氯卡班残留的新方法。方法 样品采用乙腈溶液涡旋超声提取后，加入十八烷基键合硅胶吸附剂(C18)作为分散固相萃取净化剂进行净化(200 mg)，用高效液相色谱法测定，采用PICKERING C8 色谱柱(4.6 mm×250 mm i.d.，5 μm)，以甲醇:水(78:22，V:V)的溶液为流动相等度洗脱，流速1.2 mL/min，检测波长为278 nm。结果 在最优检测条件下，各目标化合物的加标回收率为72.9%~104.4%，相对标准偏差在2.1%~9.1%之间。三氯生及三氯卡班的检出限分别为0.05和0.02 mg/kg，定量限分别为0.15和0.06 mg/kg。结论 该方法分析快速、简便，适用于水果中三氯生及三氯卡班的测定。

英文摘要:

      Objective To develop adetermination of triclosan and triclocarban in fruit by dispersive solid-phase extraction (DSPE) combined with high performance liquid chromatography (HPLC). Methods The analytes were supersonic extracted from the fruit samples by acetonitrile, and purified by dispersion solid phase extraction using C18 sorbent (200 mg), then analyzed by high performance liquid chromatography. The chromatographic separation was performed on a PICKERING C8 column (4.6 mm×250 mm i.d.，5 μm). The mobile phase consists of methanol-H2O (78:22 V:V) at a flow rate of 1.2 mL/min, the detector is set at 278 nm. Results The recoveries were in the range of 72.9%~104.4%, and the relative standard deviation(RSD)were2.1%~9.1%.The limit of detection were 0.05 and 0.02 mg/kg, and the limit of quantification were 0.15 and 0.06 mg/kg for triclosan and triclocarban．Conclusion This method was quick and easy, and applicable to determinate triclosan and triclocarban in fruit.