不同前处理方法对南美白对虾中游离甲醛 测定的影响

Effects of different pretreatments on detecting free formaldehyde in Penaeus vannamei

中文关键词:  南美白对虾  游离甲醛  超声波  前处理

英文关键词:Penaeus vannamei  free formaldehyde  ultasonic  preliminary treatment

中文摘要:

      目的 利用不同前处理方法对乙酰丙酮法测定南美白对虾(Penaeus vannamei)中游离甲醛进行了研究, 从而为水产品的中游离甲醛的提取、检测提供一定的理论基础和技术支持。方法 通过对比高氯酸和三氯乙酸分别结合超声波和静置4种提取游离甲醛的前处理方法, 对提取效果较好的前处理进行单因素和正交试验进行条件优化。结果 高氯酸结合超声波提取游离甲醛的含量测定值最高, 为1.68 mg/kg, 回收率为79.53%, 提取效果较好, 对高氯酸浓度、超声波温度和超声波时间进行正交试验进行方差分析表明3个因素对游离甲醛提取效果的影响为: 高氯酸浓度>超声波时间>超声波提取温度, 高氯酸浓度影响极显著(P<0.01), 最佳组合为: 4%高氯酸作为提取剂, 在30 ℃下超声35 min。结论 本试验研究可作为提取南美白对虾中游离甲醛的较宜的前处理方法。

英文摘要:

      Objective To determine the content of 4 different preliminary treatments of extracting free formaldehyde in Penaeus vannameis by acetylacetone spectrophotometry, and provide reference for formaldehyde extracting and detecting in fishery products. Methods Trichloroacetic acid and perchloric acid combined with ultrasonic and soaking statically to extract free formaldehyde were compared respectively. And then, the single factor experiment and orthogonal experiment were carried out to improve the extracting effect. Results It showed that perchloric acid combined with ultrasound treatment reveal the highest content of free formaldehyde, which was 1.68 mg/kg, and the recovery rate was 79.53%. By analysis of variance, perchloric acid concentration had the biggest and significantly (P<0.01) influence on free formaldehyde extracting effect, and the influence of ultrasonic time was bigger than ultrasonic temperature. As a result, the most effective method for extracting free formaldehyde in Penaeus vannamei had been found out: 4% perchloric acid as extractant, and ultrasound treatment for 35 minutes at 30 ℃. Conclusion The reasearch can be used as appropriate pretreatment method for extracting free formaldehyde in Penaeus vannamei.