云南不同产地臭灵丹草氨基酸测定及营养评价

Amino Acid Composition and Nutritional Evaluation of Laggera pterodonta（DC.） Benth. from Different Areas in Yunnan

中文关键词:  臭灵丹草  氨基酸  成分分析  营养评价

英文关键词:Laggera pterodonta（DC.）Benth.  amino acids  content analysis  nutritional evaluation

中文摘要:

      目的 本文主要以云南10个不同产地臭灵丹草为研究对象，测定氨基酸组成及含量，对其营养价值进行评价，为臭灵丹草的资源利用及质量控制提供理论依据。方法 氨基酸的测定采用氨基酸自动分析仪检测进行，并利用氨基酸比值系数法和聚类分析法探讨不同产地臭灵丹草在氨基酸营养价值上的差异。结果 各产地臭灵丹草中均含有17种氨基酸，以及1种非蛋白类氨基酸γ-氨基丁酸，其中包括7种人体必需氨基酸，各产地臭灵丹草氨基酸总量为9.25%-~19.74%，必需氨基酸含量为3.97%~-8.63%，各产地臭灵丹草中氨基酸总量和必需氨基酸含量有明显差异，但各产地臭灵丹草中各种氨基酸含量分布类似。臭灵丹草中各种人体必需氨基酸种类齐全 ，含量较高，且比例均衡，超过FAO/ WHO提出的推荐值。臭灵丹草的氨基酸比值系数分（SRC ）平均值为78.22，第一限制氨基酸为蛋氨酸+胱氨酸 。臭灵丹草中含有丰富的药效氨基酸，其占氨基酸总量的62.74%~-66.48%。以氨基酸总量、必需氨基酸含量、药效氨基酸含量、必需氨基酸与氨基酸总量比值（E/T）、必需氨基酸与非必需氨基酸比值（E/N ）、SRC值等为指标，聚类分析将各地臭灵丹草分为4类。结论 臭灵丹草具有较高的营养价值和保健作用，有待成为新资源食品，可进一步开发利用。

英文摘要:

      Objective To determinate and evaluate the nutrition quality of Laggera pterodonta（DC.）Benth. from 10 regions of Yunnan through amino acids composition and content, in Laggera pterodonta（DC.）Benth. from 10 regions of Yunnan, which in order to provide a theoretical basis for the resource utilization and quality control of Laggera pterodonta（DC.）Benth.. Methods The amino acid composition were analyzed by usingwith an automatic amino acid analyzer. Ratio coefficient of amino acid method and cluster analysis were conducted to evaluate nutrition quality and distinguish differences among the heterotrophic strains of L. pterodonta（DC.） Benth.. Results All samples were all rich in 17 amino acids and γ-Aminobutyric, including 7 essential amino acids. The total amino acids contents in L. pterodonta（DC.） Benth. from different regions ranged from 9.25% to19.74% , the essential amino acids contents ranged from 3.97% to8.63%.Although there were significant differences both of the total amino acids and essential amino acids from different regions, the distribution of amino acids contents from rich to low were similar. L. pterodonta（DC.） Benth. contained almost all essential amino acids in a well-balanced proportion, and the essential amino acid composition exceeded the FAO/WHO recommended pattern. With amino acids ratio coefficient, the score of amino acids ratio coefficient SCR C was 78.22. The first limiting amino acids were Met+Cys , the medicinal amino acids took up 62.74-%~66.48% of the total amino acids. In addition, the multivariate statistical method was used to analyze the differences in amino acid contents, essential amino acid contents, medicinal amino acid contents,the ratio of essential amino acids and total amino acids (E/T), E/N the ratio of essential amino acids and non essential amino acids( E/N )and SRC among the samples from ten regions. These geographic origins were classified into 4 categories by cluster analysis. Conclusion L. pterodonta（DC.） Benth. has high nutritional value and health benefits, and can be processed into natural green products.