赭曲霉毒素A的适配体筛选

Screening of DNA aptamer of chratoxin A

中文关键词:  真菌毒素  赭曲霉毒素  核酸适配体  指数富集的配基系统进化技术  固相亲和柱

英文关键词:Mycotoxin  ochratoxin  aptamers  SELEX  affinity column

中文摘要:

      目 的 筛选小麦和其他相关食品中常见的赭曲霉毒素A真菌毒素核酸适配体。方 法 采用固态靶标筛选策略和磁分离的筛选方法筛选赭曲霉毒素A的核酸适配体。结 果 获得的赭曲霉毒素A核酸适配体亲和力较高，解离常数为纳摩尔级；特异性好，与赭曲霉毒素A结构相似性化合物如赭曲霉毒素B、N-乙酰苯丙氨酸或华法令不结合或结合力非常弱。结 论 本文采用固态靶标筛选策略和磁分离的SELEX筛选方法获得了赭曲霉毒素A的高特异性，高亲和力核酸适配体，有望在农产品质量安全领域替代传统抗体开发真菌毒素快速检测传感器或制备固相亲和柱。

英文摘要:

      Objective Screening aptamer of ochratoxin A (OTA), a common mycotoxin in wheat and other related food. Methods We got the OTA aptamer using solid-target selection strategy and magnetic-separation selection method. Results The OTA aptamer has high affinity with nanomole dissociation constants and good specificity non-recognizing or having weak affinity towards structure similarity compounds of OTA such as Ochratoxin B (OTB), N-acetyl-L-phenylalanine and warfarin. Conclusion The OTA aptamer getting from solid-target selection strategy and magnetic-separation selection method, has high affinity and good specificity. Instead of traditional antibody, the OTA aptamer may be used to develop sensor and affinity chromatography column for mycotoxins rapid detection in the field of agricultural products and food.