液相色谱-四极杆飞行时间质谱法筛选产来氟米特芽胞杆菌菌株

Screening of Bacillus strains producing Leflunomide based on liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry

中文关键词:  液相色谱-四极杆飞行时间质谱法  芽胞杆菌  来氟米特

英文关键词:liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry  Bacillus  Leflunomide

中文摘要:

      目的 筛选产来氟米特的芽胞杆菌菌株。方法 采用液相-四级杆飞行时间质谱的方法分析了31株芽胞杆菌菌株的发酵液胞外成分。结果 在谱库扫描得到的代谢物中, 来氟米特的含量相对较高, 且与谱库的匹配率最高。筛选出含来氟米特成分的菌株为科恩芽胞杆菌 (Bacillus cohnii FJAT-10017)、嗜碱芽胞杆菌(Bacillus alcalophilus FJAT-10014)、土壤短芽胞杆菌(Brevibacillus agri FJAT-10018)、解硫胺素解硫胺素芽胞杆菌(Aneurinibacillus aneurinilyticus FJAT-10004)、蜥蜴纤细芽胞杆菌(Gracilibacillus dipsosauri FJAT-14266)。其中该成分含量最高的为蜥蜴纤细芽胞杆菌(Gracilibacillus dipsosauri FJAT-14266), 占其发酵液总代谢物相对含量的12.16 %。结论 蜥蜴纤细芽胞杆菌(Gracilibacillus dipsosauri FJAT-14266)为来氟米特高产菌株。该研究为芽胞杆菌来源的免疫调节剂的开发与利用提供理论依据。

英文摘要:

      Objective To screen Bacillus strains producing Leflunomide. Methods Extracellular metabolites of 31 Bacillus strains were analyzed by liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry (LC-QTOF MS). Results Extracellular metabolites were detected in 31 Bacillus strains. The content of Leflunomide was relatively high in the detected compounds, and with the highest match degree. The result showed that six Bacillus strains contained Leflunomide, including Bacillus cohnii FJAT-10017, Bacillus alcalophilus FJAT-10014, Brevibacillus agri FJAT-10018, Aneurinibacillus aneurinilyticus FJAT-10004, and Gracilibacillus dipsosauri FJAT-14266. The content of Leflunomide was highest in Gracilibacillus dipsosauri FJAT-14266, with the relative content of 12.16 %. Conclusion Gracilibacillus dipsosauri FJAT-14266 was the high-yield Bacillus strain producing lefunomide. This result provided the theory basis for development and utilization of this immunomodulator from Bacillus.