液相色谱串联质谱-同位素内标法快速测定 玉米面中的伏马菌毒素

Determination of fumonisins in corn by liquid chromatography-tandem mass spectrometry with isotope dilution method

中文关键词:  伏马菌素  玉米面  液相色谱-串联质谱法  同位素稀释  多功能柱

英文关键词:fumonisin  corn  liquid chromatography-tandem mass spectrometry  isotope dilution  multifunction columns

中文摘要:

      目的 建立液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry, LC-MS/MS)以同时测定玉米中3种伏马菌素(FB1, FB2 and FB3)。方法 试样经50%乙腈/水溶液超声提取, 离心, 多功能柱净化后液相色谱/串联质谱仪直接测定, 13C34-FBs内标法定量。结果 加标回收率范围为75.9%~108.2%, 方法检测限在0.39~0.58 μg/kg之间。将本方法用于实际样品分析, 67%的玉米面中同时检出3种伏马菌素(FB1, FB2 and FB3)。结论 此方法简便快速, 灵敏度较高, 可以用于准确定量检测粮食中的伏马菌素。

英文摘要:

      Objective To establish a simple and rapid method for the determination of fumonisin (FB1, FB2 and FB3) in corn by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods Homogenized samples were sequentially extracted by 50% acetonitrile-water, centrifuge, multifunction columns cleanup, evaporate, re-dissolve and filtration before analysis. Quantification of fumonisin was based on the use of 13C34-fumonisin. Results The present method showed adding standard recoveries of 75.9%~108.2%, with limit of detections (LODs) of FB1, FB2 and FB3 ranging from 0.39 to 0.58 μg/kg. Analysis of 18 corn samples by the current method was successfully performed. The simultaneous occurrence of FB1, FB2 and FB3 was observed in 67% of samples. Conclusion This quantitative method is demonstrated to be rapid, simple, and high sensitive, which can be applied in the accurate determination of fumonisin in corn.