| 刘名扬,郭 祯,赵景红,朱程云,刘 月.利用反相阳离子交换混合模式色谱同时检测10种β-内酰胺类抗生素[J].食品安全质量检测学报,2015,6(10):4135-4141 |
| 利用反相阳离子交换混合模式色谱同时检测10种β-内酰胺类抗生素 |
| Application of reverse-phase cation exchange mixed-mode chromatography detection for the simultaneous determination of β-lactam antibiotics |
| 投稿时间：2015-07-30 修订日期：2015-10-11 |
| DOI： |
| 中文关键词: 混合模式色谱 反相/弱阳离子交换混合模式 β-内酰胺类抗生素 |
| 英文关键词:mixed-mode chromatograpy reversed-phase liquid chromatography/ weak cation exchange (RPLC/WCX) β-lactam antibiotics |
| 基金项目:国家自然科学基金资助(21177040)Fund: Supported by the National Natural Science Foundation of China (21177040)*通讯作者：刘名扬,博士,研究员,教授. E-mail: liumy@mail.tsinghua.edu.cn *Corresponding author: LIU Ming-Yang, Doctor, Researcher, Professor. E-mail: liumy@mail.tsinghua.edu.cn ,2,3,*, GUO Zhen1, ZHAO Jing-Hong2, ZHU Cheng-Yun1,LIU Yue1 |
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| Author | Institution |
| liu mingyang | dalian jiaotong university |
| Guo Zhen | College of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian |
| Zhao Jing-Hong | Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian |
| Zhu Cheng-Yun | College of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian; Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian ; Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian |
| Liu Yue | College of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian |
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| 中文摘要: |
| 建立了反相/弱阳离子交换混合模式色谱(C18/WCX)对10种β-内酰胺类抗生素(头孢羟氨苄、头孢替唑钠、头孢呋辛钠、头孢唑啉、头孢拉定、头孢西酮、头孢孟多酯钠、头孢噻吩、哌拉西林、氯唑西林钠)同时检测的方法。以乙腈/甲酸铵/水为流动相,与常规反相色谱分析Unitary C18为对照,结果表明,中性条件下优化流动相洗脱梯度,10种β-内酰胺类抗生素在C18/WCX具有较高的分离选择性。通过缓冲盐浓度和pH值的调控,发现C18/WCX与样品间分离机制由反相作用力与离子交换两种作用力共同作用。研究表明,混合模式色谱在β-内酰胺类抗生素的分离分析中具有较大的潜力。 |
| 英文摘要: |
| Reversed-phase liquid chromatography/weak cation exchange (RPLC/WCX, C18/WCX) was established for the simultaneous determination of ten β-lactam antibiotics (Cefadroxil, Ceftezole sodium, Cefuroxime sodium, Cefazolin, Cephradine, Cefazedone, Cemandil sodium, Cephalothin, Pipracil and Cloxacillin sodium). A chromatographic separation was achieved on a C18/WCX with the mobile phase consisting of acetonitrile and ammonium formate mixture in a gradient mode. Compared with the conventional reversed-phase chromatography Unitary C18, the selectivity of C18/WCX can be improved. It was found that the mechanism of samples separated on C18/WCX were hydrophobic interaction and ion exchange interaction by regulated by the salt concentration and pH value. The results showed that the mixed-mode chromatography would be a potent approach for the analysis and separation of β-lactam antibiotics. |
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