抑制性电导检测-离子色谱法快速测定 水产品中的生物胺

Determination of biogenic amines in seafood by suppressed conductance de-tection ion chromatography

中文关键词:  离子色谱法  抑制性电导检测  生物胺  水产品

英文关键词:ion chromatography  suppressed conductance detection  biogenic amines  seafood

中文摘要:

      目的 建立抑制性电导检测-离子色谱法快速测定水产品中常见的几种生物胺。方法 以甲基磺酸(MSA)为淋洗液通过梯度洗脱分离目标生物胺, 采用Ionpac CS17(4 mm×250 mm)分析柱和电导检测器进行分析。进样体积为25 μL, 采用峰面积定量。通过比较不同的提取剂(TCA(三氯乙酸)、HClO4、MSA)和提取方式(振荡、超声)的提取效果优化前处理方法。结果 使用MSA超声提取效果较好, 该方法测定生物胺的线性范围为0.02 mg~10.0 mg, 检出限在0.5 mg/kg以下, 加标回收率分别为85.2%~106.9%。结论 该方法前处理简单快捷, 结果灵敏、准确, 适用于水产品中常见生物胺的快速检测。

英文摘要:

      Objective To establish a method for the determination of common biogenic amines in seafood by ion chromatography method with suppressed conductance detection. Methods The samples were analyzed by Ionpac CS17(4 mm×250 mm) column and a conductance detector, with methylsulphonic acid (MSA) as the eluent to separate the amines in a gradient elution procedure. The injection loop was 25μL, and peak area was used for quantification. In this paper, different extracting agents (TCA (trichloroacetic acid), HClO4 and MSA) and methods (shaking and sonication) were applied for the biogenic amine extraction, and the outcomes were compared and optimized. Results The sonication operation with MSA as extracting solution was proved to be the optimal pretreatment way. The linear range of the method was 0.02 mg~10.0 mg, the detection limit was below the 0.5 mg/kg, and the recovery of the amines ranged from 85.2%~106.9%. Conclusion The pretreatment of this method is simple and rapid, and the result is sensitive and precise, which makes it suitable for the application in biogenic amines determination in seafood.