高效液相色谱法同时测定果蔬中5种植物生长 调节剂残留

Determination of 5 plant growth regulators residues in fruits and vegetables by high performance liquid chromatography

中文关键词:  高效液相色谱法  果蔬  植物生长调节剂  残留检测

英文关键词:high performance liquid chromatography  fruits and vegetables  plant growth regulator  residues detection

中文摘要:

      目的 建立同时测定果蔬中噻苯隆、氯吡脲、噻唑隆、多效唑和烯效唑5种植物生长调节剂残留的高效液相色谱分析方法。方法 样品中的植物生长调节剂采用乙腈作为提取溶剂经高速匀浆提取, 经NH2固相萃取小柱净化, 用乙腈: 10 mmol/L的乙酸铵溶液(V:V, 55:45)为流动相, 选择Shiseido CAPCELL PAK MG ⅡC18色谱柱(4.6 mm×250 mm i.d., 5 μm)分离后, 检测波长为225 nm。结果 5种植物生长调节剂在0.05~1.0 mg/kg范围内具有良好的线性关系, 相关系数r≥0.995; 检出限为0.008~0.01 mg/kg, 定量限为0.020~0.032 mg/kg。在0.10、0.20、0.50 mg/kg添加水平, 5种植物生长调节剂回收率在73.8%~113.7%之间, 相对标准偏差在4.68%~9.92%之间。结论 该方法准确性、重复性、稳定性及选择性好, 能对果蔬中5种植物生长调节剂残留实现准确定量分析。

英文摘要:

      Objective To develop a method for determination of thidiazuron, forchlorfenuron, methabenzthiazuron, paclobutrazol and uniconazole residues in fruits and vegetables by high performance liquid chromatography with solid phase extraction. Methods The plant growth regulators residues in the fruits and vegetables samples were extracted by acetonitrile, cleaned up by LC-NH2 solid phase extraction, and then analyzed by using high performance liquid chromatography ultraviolet detector (225 nm) with acetonitrile and 10 mmol/L ammonium acetate (V:V, 55:45) as gradient elution solvent, with Shiseido CAPCELL PAK MG ⅡC18 (4.6 mm×250 mm i.d., 5 μm) as the analytical column. Results The recoveries of 3 additive concentrations were ranged from 73.8% to 113.7%, and the relative standard deviations (RSD) were 4.68%~9.92%. The calibration curves were in the range of 0.05~1.0 mg/kg with the correlation coefficients of more than 0.995. The limits of detection were from 0.008 to 0.01 mg/kg and the limits of quantitation were from 0.02 to 0.032 mg/kg of 5 plant growth regulators residues. Conclusion The method showed a high selectivity, good repeatability, accuracy and stability, and could be applied in the accurate quantification of 5 plant growth regulators residues in fruits and vegetables.