应用实时荧光PCR技术量化检测羊肉中猪源性和鸡源性成份

Detection and quantification pork and chicken in sheep products using real-time PCR

中文关键词:  荧光实时定量PCR  定量检测  羊肉  猪肉  鸡肉

英文关键词:real-time PCR  quantitative analysis  sheep  pork  chicken

中文摘要:

      为了经济利益，一些商贩会在羊肉中掺混其他肉类。为了实现羊肉中猪源性成份和鸡源性的定量检测，本研究以新鲜羊、猪和鸡瘦肉为样本提取DNA分子作为检测模板，针对基因组中单拷贝基因设计特异性的引物和探针，应用荧光实时定量PCR技术对模板DNA进行扩增。通过绘制扩增标准曲线和确定羊、猪和鸡的质量与DNA比值常数，对四种不同掺混比例的混合肉样进行定量分析。结果显示，检测质量百分比的绝对误差可以控制在7%以内，本研究结果可以为我国肉类食品安全监控提供技术支撑。

英文摘要:

      Due to economic reasons，pork and chicken were blended into sheep products. In this research, a real-time PCR method was provided for quantitative analysis the chicken and pork blending in sheep. The primers were designed according to the sequence of the single copy gene. A series of genome DNA were used as standard curve in real-time PCR. The mathematical conversion parameters about the mass of meat and DNA were determined. The weight/weight equivalents of four blending samples were analyzed. The results showed that the absolute error of detection with a tolerance of less than 7%. This research can provide technical support for food safety.