HPLC法测定鲜切富士苹果中多酚类物质的研究

Quantitative determination of phenolics composition in fresh-cut ‘Fuji’ apples by high performance liquid chromatography

中文关键词:  鲜切苹果  高效液相色谱法  多酚

英文关键词:fresh-cut apples  high performance liquid chromatography  phenolics

中文摘要:

      目的 分析鲜切富士苹果中多酚成分及含量。方法 鲜切苹果多酚采用80%乙醇超声提取, 利用高效液相色谱法法分析鲜切苹果中多酚成分, 外标法定量分析各多酚组分的含量。结果 10种多酚标准品的溶液浓度和检测响应值呈现出良好的线性关系, 其相关系数r2均大于0.99, 且回收率较超过95%, 重复性好。鲜切富士苹果中主要含有绿原酸、表儿茶素、儿茶素、丁香酸和咖啡酸5种多酚物质, 其中绿原酸和表儿茶素含量较高, 分别为101.02±0.31 μg/g和100.80±2.65 μg/g。结论 鲜切富士苹果富含具有抗氧化活性的多酚成分。

英文摘要:

      Objective To analyze the phenolics composition of fresh-cut ‘Fuji’ apples. Methods Phenolics were extracted from fresh-cut apples with 80% ethanol. Phenolics composition was analyzed by high performance liquid chromatography (HPLC). An external standard method was used for quantitative analysis. Results The results showed that 10 polyphenols could be separated and the calibrations curves were linear with r2>0.999 and the recovery rate was above 95%. HPLC analysis revealed that the main phenolic compounds in fresh-cut ‘Fuji’ apples were chlorogenic acid followed by chlorogenic acid, epicatechin, catechin, syringic acid and caffeic acid. The concentrations of chlorogenic acid and epicatechin were higher than others, with the values 101.02±0.31 μg/g and 100.80±2.65 μg/g, respectively. Conclusion Fresh-cut apple is rich in polyphenols with antioxidant activity.