高效液相色谱-串联质谱法测定蜂胶软胶囊中 黄曲霉毒素B1

Determination of aflatoxin B1 in propolis soft capsules by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  黄曲霉毒素B1  蜂胶软胶囊  保健食品  高效液相色谱-串联质谱法

英文关键词:aflatoxin B1  propolis soft capsules  health food  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立测定蜂胶软胶囊中黄曲霉毒素B1的高效液相色谱-串联质谱法。方法 样品经甲醇超声提取,用氰基柱将黄曲霉毒素B1分离, 用高效液相色谱-串联质谱进行测定, 外标法定量。结果 黄曲霉毒素B1标准曲线在0.1~2.0 ng/mL范围内有良好的线性, r>0.9998, 回收率为80.1%~87.8%, RSD<3.0%, 检出限为1.0 μg/kg。结论 该方法结果准确可靠, 较节省试剂、耗材, 节省样品前处理时间, 有利于降低检验成本, 适用于保健食品蜂胶软胶囊中黄曲霉毒素B1的限量测定。

英文摘要:

      Objective To establish a method for determination of aflatoxin B1 in propolis soft capsules by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods Samples were ultrasonically extracted with methanol, and aflatoxin B1 were separated with the cyano column by high performance liquid chromatography-tandem mass spectrometry, then measured by external standard method. Results Aflatoxin B1 standard curve had a good linear relationship within 0.1~2.0 ng/mL (r>0.9998), the recovery was 80.1%~87.8%, RSD<3.0%, and the limit of quantification was 1.0 μg/kg. Conclusion The established method is accurate and reliable, and it can save the pretreatment time of sample and reduce the test cost. It is suitable for the limited determination of aflatoxin B1 in propolis soft capsules of health food.