高效液相色谱-串联质谱法测定保健食品中 黄曲霉毒素M1

Determination of aflatoxin M1 in health food by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  黄曲霉毒素M1  保健食品  高效液相色谱-串联质谱法

英文关键词:aflatoxin M1  health food  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立测定保健食品中黄曲霉毒素M1的高效液相色谱-串联质谱法。方法 样品经甲醇超声提取, 用氰基柱将黄曲霉毒素M1分离, 用高效液相色谱-串联质谱进行测定, 外标法定量。结果 黄曲霉毒素M1标准曲线在0.1~2.0 ng/mL范围内有良好的线性关系, r>0.9998, 回收率为77.5%~97.8%, RSD<5.0%, 检出限为0.1 μg/kg。结论 该方法结果准确可靠, 可节省样品前处理时间, 有利于降低检验成本, 适用于保健食品中黄曲霉毒素M1的限量测定。

英文摘要:

      Objective To establish a method for determination of aflatoxin M1 in health food by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The samples were ultrasonic extracted with methanol, and aflatoxin M1 were separated with the cyano column by high performance liquid chromatography-tandem mass spectrometry, then detected by external standard method. Results Aflatoxin M1 standard curve had a good linear relationship within 0.1~2.0 ng/mL (r＞0.9998), the recovery was 77.5%~97.8%, RSD＜5.0%, and the limit of quantification was 0.1 μg/kg. Conclusion The established method is accurate and reliable, and it can save the pretreatment time of sample and reduce the test cost. It is applicable for the determination of aflatoxin M1 in health food.