反相高效液相色谱法测定油类保健食品中 混合生育酚

Determination of mixed tocopherols in oil health food by reversed-phase high performance liquid chromatography

中文关键词:  混合生育酚  保健食品  反相高效液相色谱法  维生素E

英文关键词:mixed tocopherols  health foods  reversed-phase high performance liquid chromatography  vitamin E

中文摘要:

      目的 建立测定油类保健食品中混合生育酚的反相高效液相色谱法。方法 样品经氢氧化钾甲醇溶液皂化, 盐酸甲醇溶液中和; 用反相高效液相色谱: 色谱柱(SUPELCO, C18, 4.6 mm×150 mm, 5 μm); 流速: 1.0 mL /min; 柱温: 40 ℃; 进样量: 20 μL; 波长: 294 nm; 流动相: 水和甲醇, 梯度洗脱; 将α-、γ-及δ-生育酚分离; 外标法定量。结果 本方法标准曲线在0.01~0.20 mg/mL范围内有良好的线性关系, r>0.9990, 回收率为93.5%~103.8%, RSD<4.0%, 检出限为0.02 μg/kg, 定量限为0.1 μg/kg。结论 该方法结果准确可靠, 节省样品前处理时间及试剂, 有利于降低检验成本, 适用于油类保健食品中混合生育酚含量的测定。

英文摘要:

      Objective To establish a method for determination of mixed tocopherols in oil health foods by reversed-phase high performance liquid chromatography. Methods The samples were saponified by potassium hydroxide methanol, and neutralized by hydrochloric acid methanol. The extracts were separated by RP-HPLC with the column of SUPELCO, C18 (4.6 mm×150 mm, 5 μm), flow rate of 1.0 mL/min, column temperature of 40 ℃, injection volume of 20 μL, wavelength of 294 nm, mobile phase of water and methanol, and with gradient elution to separate α-, γ-and δ-tocopherol; then were determined by external standard method. Results Tocopherols standard curve had a good linear relationship within 0.01~0.20 mg/mL range (r>0.9990), the recovery was 93.5%~103.8%, RSD＜4.0%, limit of determination was 0.02 μg/kg, and the limit of quantity was 0.1 μg/kg. Conclusion The method is accurate and reliable, saving pretreatment time and reagents, can reduce the test cost; it is suitable for the determination of mixed tocopherol in oil health food.