间接竞争ELISA检测试剂盒测定粮油食品中的 黄曲霉毒素B1

Determination of aflatoxin B1 in cooking oil, peanut, and grain by using direct competitive ELISA kit

中文关键词:  黄曲霉毒素B1  酶联免疫试剂盒  高效液相色谱法

英文关键词:aflatoxin B1  enzyme-linked immunosorbent assay kit  high-performance liquid chromatography

中文摘要:

      目的 建立一套适用于食品中黄曲霉毒素B1的快速、简便、准确的检测方法, 同时, 进一步验证本公司研制的黄曲霉毒素ELISA检测试剂盒的检测效果。方法 用酶联免疫法和高效液相色谱法分别对市售的食用油、花生、谷物样品中AFB1的污染程度进行检测分析。结果 用ELISA法和HPLC法测定食用油样品的回收率分别为87.1%～92.7%和85.8%～100.8%; 花生样品的回收率分别为76.0%～92.8%和76.3%～92.9%; 谷物样品的回收率分别为82.6%～92.7%和82.7%～106.4%, 花生加标样品6次平行测定的RSD分别为1.29%～2.46%和5.15%～8.70%, 11份阳性样品测定结果表明2种方法具有很好的一致性(r2=0.995)。结论 ELISA法和HPLC法均有很好的线性关系, 且测定结果相近。本公司研制的黄曲霉毒素ELISA试剂盒可以快速地检测食品中黄曲霉毒素B1, 分析周期短, 分析效率高。

英文摘要:

      Objective To establish a set of rapid, simple, and accurate method for aflatoxin B1 in food. At the same time, to further verify the test effect of aflatoxin ELISA kit developed by Beijing Kwinbon biotechnology company. Methods To investigate the occurrence of aflatoxin B1 in cooking oil , peanut, and grain by using direct competitive enzyme-linked immunosorbent assay (ELISA) kit and high-performance liquid chromatography (HPLC). Results The recovery rates of AFB1 from the cooking oil spiked by ELISA and HPLC were 87.1%~92.7% and 85.8%~100.8%, respectively. The recovery rates of AFB1 from the peanut spiked by ELISA and HPLC were 76.0%～92.8% and 76.3%～92.9%, respectively. The recovery rates of AFB1 from the grain by ELISA and HPLC were 82.6%～92.7% and 82.7%～106.4%, respectively. The RSD of 2 methods from the peanut sample by 6 parallel determinations were 1.29%~2.46% and 5.15%~8.70%. By 11 positive samples results showed that those two methods had a very good consistency (r=0.995). Conclusion It showed that the linear calibration for ELISA and HPLC were good. The measurement results of these methods were close with two methods. The ELISA kit which developed by Beijing Kwinbon Biotechnology company could simultaneously determine aflatoxin B1 in food, and had advantages of short period and high efficiency of analysis.