高效液相色谱法测定维生素片中维生素A醋酸酯含量的不确定度评定

Assessment of content uncertainty of vitamin A acetate in vitamin tablets by high performance liquid chromatography

中文关键词:  高效液相色谱法  维生素A醋酸酯  数学模型  不确定度

英文关键词:high performance liquid chromatography  vitamin A acetate  mathematical model  uncertainty

中文摘要:

      目的 分析高效液相色谱法测定维生素片中维生素A醋酸酯含量的不确定度来源和影响不确定度的因素, 为简化评定方法和提高检测质量提供依据。方法 根据检测过程, 建立数学模型, 分析影响不确定度因素的来源, 通过对不确定度进行量化分析, 得出扩展不确定度。结果 由各不确定度分量评定结果可以看出, 高效液相色谱法测定维生素片中维生素A醋酸酯含量的扩展不确定度为0.46 mg/100 g, 平均含量为35.72 mg/100 g, 包含因子k=2。结论 高效液相色谱法测定维生素片中维生素A醋酸酯含量的不确定度主要由标准溶液配制、重复测定和稀释标准溶液引入。因此在含量检测过程中, 要控制各不确定因素, 以提高检测准确性。

英文摘要:

      Objective To analyze the sources and influencing factors of content uncertainty of vitamin A in vitamin tablets by high performance liquid chromatography (HPLC), so as to simplify the evaluation method and increase the testing quality. Methods A mathematical model was established to analyze the sources and influencing factors in uncertainty according to the testing process, and through the quantitative analysis of uncertainty, the expanded uncertainty was obtained. Results The expanded uncertainty of vitamin A in vitamin tablets by HPLC was 0.46 mg/100 g, the average content was 35.72 mg/100 g, and the including factor k=2 through evaluation of the results in uncertainty components. Conclusion The main sources of uncertainty of vitamin A in vitamin tablets by HPLC were preparing the standard solution, repetitive measurement, and thinning the standard solution. Therefore, it was necessary to control various uncertain factors in order to improve detection accuracy in the testing process.