食源性致病菌快速检测技术研究进展

Research progress on rapid detection of foodborne bacterial pathogens

中文关键词:  食源性致病菌  快速检测技术  免疫学  分子生物学

英文关键词:foodborne bacterial pathogens  rapid detection  immunology  molecular biology

中文摘要:

      食源性致病菌快速检测技术的发展对于我国应对和控制食源性疾病的爆发至关重要。目前在基层监管执法中应用最为广泛的食源性致病菌快速检测技术主要包括以免疫学为基础的酶联免疫吸附技术和免疫磁珠分离技术; 以及以分子生物学技术为基础的PCR技术和环介导等温扩增等技术。本篇综述对上述技术的检测特点和应用情况进行了深入的分析, 同时, 还对在食源性致病菌快速检测领域极具有发展前景的双功能抗体检测技术和变性高效液相色谱分析技术进行了详细的介绍, 最后文章分析了目前食源性致病菌快检的技术瓶颈主要在于所有快检技术的建立很难绕过致病菌富集和分离的过程, 这一步骤很大程度上延长了食源性致病菌快检所需的时间。研发高效、快速的致病菌富集分离技术是真正实现食源性致病菌快速检测的关键。

英文摘要:

      Rapid detection of foodborne bacterial pathogens is the key to prevent and control food-borne disease in China. Immunology based ELISA (enzyme linked immunosorbent assay) and IMS (immunomagnetic separation), and molecular biology technology based PCR and LAMP (loop-mediated isothermal amplification) were the most popular rapid detection of foodborne pathogenic bacteria in routine work at primary regulatory authority. The application and detection feature were analyzed in-depth in this manuscript. Bi-functional antibodies assay and DHPLC (denaturing high performance liquid chromatography) also were discussed in this review as a kind of technologies which had a good development prospects. The procession of enrichment and separation during the food-borne pathogens bacterial detection was hard to avoid. These steps greatly extended the required time of detection. The development of efficient and rapid enrichment and separation method is the key technology for the rapid detection of food-borne pathogenic bacteria.