青海春油菜籽皮原花青素的提取工艺优化

Technology optimization for extracting proanthocyanidin from spring rapeseed hull in Qinghai province

中文关键词:  原花青素  油菜籽皮  提取  响应面分析  工艺优化

英文关键词:proanthocyanidin  rapeseed hull  extraction  response surface analysis  technology optimization

中文摘要:

      目的 为了提高春油菜籽皮原花青素的提取率, 对青海春油菜籽皮原花青素提取的方法进行研究, 对提取的关键工艺参数进行优化。方法 采用单因素试验探讨了乙醇浓度、提取温度、提取时间及料液比对原花青素提取效果的影响, 并运用Box-Behnken中心组合试验分析乙醇浓度、提取温度和提取时间3个因素对提取原花青素的影响, 采用响应曲面分析法优化提取工艺。结果 最佳工艺条件为: 乙醇浓度63.7%、浸提温度58.9 ℃、浸提时间53 min。在该条件下, 提取粗原花青素质量比的预测值为5.16 mg/g, 验证试验值为5.22 mg/g。通过响应面分析法优化了春油菜籽皮原花青素的提取工艺, 在最佳提取工艺条件下进行验证试验得到的结果与预测理论值相近。结论 响应曲面分析法优化油菜籽皮中原花青素提取工艺是可行的。

英文摘要:

      Objective To increase the yield of extracting proanthocyanidin from rapeseed skin, the methods of extraction were studied, and the technology of extraction was optimized. Methods The single factor tests of ethanol volume fraction, extraction temperature and extraction time were studied to explore the effect on extraction of proanthocyanidin in rapeseed skin. The optimum extraction processing was evaluated by Box-Behnken central composite experimental design and response surface methodology analysis. Results The optimum extraction conditions were that ethanol volume fraction was 63.7%, extraction temperature was 58.9 ℃ and extraction time was 53 min. Under these optimized conditions, the experimental value of proanthocyanidin extraction yield was 5.16 mg/g, which was exactly in accordance to the predicted value 5.22 mg/g. The proanthocyanidin extracted process of spring rapeseed skin was optimized by response surface methodology, while validate test results were close to theoretical values under the optimum extraction conditions. Conclusion The extraction proanthocyanidin from rapeseed hull using response surface methodology is feasible and reliable.