高压脉冲电场技术制备大豆肽螯合钙

Preparation of soybean protein hydrolysates chelating calcium with pulses electric field technology

中文关键词:  蛋壳  大豆肽螯合钙  高压脉冲电场

英文关键词:eggshell  soybean protein hydrolysates chelating calcium  pulses electric field

中文摘要:

      目的 研究大豆肽螯合钙最佳工艺参数以及高压脉冲电场对螯合效果的影响。方法 以鸡蛋壳粉和大豆肽粉为原料, 以螯合率为衡量指标, 研究了大豆肽粉和蛋壳粉的质量比、反应体系pH、螯合过程中电场强度以及电场的频率等因素对螯合反应的影响情况, 并利用响应面试验设计的方法。结果 回归方程为: Y=94.96-0.074X1+0.30X2-0.14X3-0.45X4+0.012X1X2+0.060X1X3+0.37X1X4-0.32X2X3+0.64X2X4-0.085X3X4-0.42X12- 0.93X22-0.34X32-0.15X42, 优化出大豆肽螯合钙的最佳工艺条件为: 大豆肽粉和蛋壳粉质量比1:1.75、pH 8.0、电场强度15 kV/cm、电场频率1200 Hz。在此条件下, 螯合率高达95.38?Ee0.18%。结论 本研究为大豆肽螯合钙技术研究提供一种新思路。

英文摘要:

      Objective To research the key technology of chelation reaction of soybean protein hydrolysates chelating calcium, and the improvement of chelating rate with the pulses electric field(PEF) as an assistant method. Methods Eggshell and soybean protein hydrolysates were used as raw material, and chelating rate was used as testing indexes. To optimize chelation rate of the soluble complexes formed during calcium binding by soybean protein hydrolysates, 4 important parameters (pH value, temperature, time and mass ratio) were examined in this experiment. Results According to one-factor-at-a-time and response surface experiment, the regression model was: Y=94.96-0.074X1+0.30X2-0.14X3-0.45X4+0.012X1X2+0.060X1X3+ 0.37X1X4 -0.32X2X3+0.64X2X4-0.085X3X4-0.42X12-0.93X22-0.34X32-0.15X42, the optimum preparation conditions of soybean protein hydrolyzates product chelating with calcium were obtained as follows: soybean protein hydrolyzates/calcium mass ratio of 1:1.75, pH 8.0, electric field intensity 15 kV/cm, and frequency 1200 Hz. Under these conditions, the chelation rate achieved to 95.38?Ee0.18%. Conclusion A scientific basis was provided for soluble complexes formed during calcium binding by soybean protein hydrolysates.