黄曲霉毒素M1 ELISA试剂盒的检测效果研究

Research on detection results of aflatoxin M1 enzyme-linked immune sorbent assay detection kit

中文关键词:  黄曲霉毒素M1  酶联免疫法  高效液相色谱法  牛奶

英文关键词:aflatoxin M1  enzyme linked immune sorbent assay  high performance liquid chromatography  milk

中文摘要:

      目的 为验证本公司生产的黄曲霉毒素M1酶联免疫法 (ELISA)检测试剂盒的检测效果。方法 用ELISA检测试剂盒对牛奶、酸奶、奶粉和干酪等4种样品中黄曲霉毒素M1的残留量进行检测, 并与高效液相色谱荧光检测法进行对照。结果 该试剂盒对4种样品的最低检测限分别为0.039、0.192、0.306、0.199 μg/kg(L), 试剂盒板内板间变异系数均小于5%; 对4种样品做加标回收试验, 其回收率均在93%~120%之间, 变异系数均小于10%; 该方法与高效液相色谱法检测实际样品的阴、阳性判断结果一致。结论 该方法稳定、可靠、灵敏, 可满足食品中黄曲霉毒素M1残留快速检测的需要。

英文摘要:

      Objective To verify the test effect of aflatoxin M1 enzyme-linked immune sorbent assay (ELISA) detection kit developed by Beijing Kwinbon biotechnology company. Methods The method of ELISA was used to detect the residues of aflatoxin M1 in milk, yoghurt, milk powder and cheese, and compared with high performance liquid chromatography (HPLC). Results The results showed that the minimum detection limitation of the kit for the 4 kinds of samples were 0.039, 0.192, 0.306, and 0.199 μg/kg (L), the coefficient of variation was less than 5%. The recovery of the method was in the range of 93%~120%, and the coefficient of variation was less than 10%. The judgments of positive and negative by ELISA were the same as those of HPLC. Conclusion This method was efficient, reliable and sensitive, and could meet the fast-test for the determination of aflatoxin M1 residues in food.