反相高效液相色谱和超高效液相色谱法测定 饮料中咖啡因含量的比较研究

Comparison of reversed high performance liquid chromatography and ul-tra-high performance liquid chromatography on quantitative determination of caffeine in beverages

中文关键词:  反相高效液相色谱法  超高效液相色谱法  饮料  咖啡因

英文关键词:reversed high performance liquid chromatography  ultra-high performance liquid chromatography  beverages  caffeine

中文摘要:

      目的 对原反相高效液相色谱(RP-HPLC)方法进行转化并优化, 建立超高效液相色谱(UPLC)方法, 为饮料中咖啡因的含量测定提供更快速、高效、灵敏和环保的方法。方法 分别采用RP-HPLC法和UPLC法测定饮料中咖啡因的含量, 并对比二者的精密度、稳定性、重复性和加标回收率。结果 2种方法所得8种市售饮料中咖啡因的含量测定结果变化趋势一致, 但UPLC法含量测定结果比RP-HPLC法含量测定结果偏高, 二者精密度、回收率均符合要求。结论 UPLC法可以成功替代RP-HPLC法, 为饮料中咖啡因的快速定性定量分析与检测提供新的途径。

英文摘要:

      Objective To establish an ultra performance liquid chromatography (UPLC) method to replace the reversed high performance liquid chromatography (RP-HPLC) for a faster, more sensitive and more environmental friendly detection for caffeine content in beverages. Methods Both RP-HPLC and UPLC methods were used in the determination of caffeine content in 8 kinds of beverages, and the precision, stability, repeatability and recovery rates of the 2 methods were compared. Results Caffeine content determined in 8 beverages by the 2 methods had a similar trend, but the content of caffeine determined by UPLC was higher than that by RP-HPLC. Both methods showed good precision and recovery rates. Conclusion UPLC method as a new detection method can replace RP-HPLC in the quantitative analysis of caffeine in beverages.