液相色谱法测定保健食品中硫酸软骨素的含量

Determination of chondroitin sulfate in health food by high performance liquid chromatography

中文关键词:  高效液相色谱法  保健食品  硫酸软骨素  含量测定

英文关键词:high performance liquid chromatography  health foods  chondroitin sulfate  determination

中文摘要:

      目的 建立一种快速、简便测定保健食品中硫酸软骨素的液相色谱方法, 并进行方法学研究。方法 样品经过前处理, 以乙腈和0.005 mol/L戊烷磺酸钠溶液为流动相, 等度洗脱, 经Diamonsil C18柱分离, 于192 nm波长检测。结果 用本方法进行检测, 能很好地分离硫酸软骨素, 在浓度0.008 mg/mL~0.04 mg/mL之间呈现良好的线性关系, 方法的相关系数可达1.000, 平均回收率在98.5%~100.0%之间, 相对标准偏差为0.6%。结论 采用该方法能测定保健食品中硫酸软骨素, 耗时短、操作简便、准确、重现性好, 分离效果明显, 可在实验室推广。

英文摘要:

      Objective To investigate a quick and simple method for determination of chondroitin sulfate in health foods by high performance liquid chromatography (HPLC). Methods Samples were treated using 0.005 mol/L pentane solution of sodium-acetonitrile as mobile phase with isocratic elution, separated on diamonsil C18 column, and detected at 192 nm wavelength. Results Chondroitin sulfate could be separated well by this method. It had a good linear relationship within 0.008~0.04 mg/mL (r=1.000), the average recoveries was 98.5%~100.0%, and the RSD was 0.6%. Conclusion The method can be used for determination of chondroitin sulfate in health foods, it is quick, simple and accurate, and has a good reproducibility and clear separation, which can be popularized in the laboratory.