朝鲜蓟叶水提取物对酒精诱导人肝癌细胞株HepG2损伤的影响

Effects of artichoke (Cynara scolymus L.) leaf extractive on alcohol-induced injury of HepG2 cell

中文关键词:  朝鲜蓟叶水提取物  HepG2细胞  酒精性肝细胞损伤

英文关键词:artichoke leaf extractive  HepG2 cells  alcohol-induced liver cell damage

中文摘要:

      目的 通过建立酒精损伤HepG2细胞模型, 探讨朝鲜蓟叶水提取物对酒精诱导的HepG2细胞损伤的影响。方法 以人肝癌细胞株HpeG2为实验材料, 采用酒精加入细胞培养液培养HepG2细胞, 建立酒精损伤HepG2细胞模型, 以MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide)法筛选酒精处理浓度、时间以及朝鲜蓟叶水提取物最佳作用浓度, 通过检测各组细胞培养基中谷丙转氨酶(alanine aminotran-sferase, ALT)、谷草转氨酶(aspartate transaminase, AST)和细胞内超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malondialdehyde, MDA)、甘油三酯(triglyceride, TG)的活性, 来评价朝鲜蓟叶水提取物对酒精诱导的HepG2肝细胞损伤的影响。结果 分别以0.6%、1.2%、2.4%的酒精浓度联合2.5、5、10 μg/mL朝鲜蓟叶水提取物培养HepG2细胞48 h。模型组随着酒精浓度增高, 细胞的AST、ALT、TG、MDA水平明显升高, SOD活性显著下降。处理组随着朝鲜蓟叶水提取物使用浓度的增加, 细胞中AST、ALT、TG、MDA水平与模型组相比明显下降, SOD活性升高, 对HepG2细胞的保护作用增强。结论 朝鲜蓟叶水提取物在一定程度上能保护酒精受损的HepG2细胞, 具有深入研究和开发的潜能。

英文摘要:

      Objective To investigate the effect of artichoke (Cynara scolymus L.) leaf extract on alcohol-induced HepG2 cell damage by the establishment of alcohol damage HepG2 liver cell model. Methods Alohol-induced HepG2 cell damage model was established by adding alcohol to the cell culture medium of HepG2 cell and using hepatoma cell line. MTT(3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay was used to determine the optimal alcohol concentration, treating time and the artichoke leaf extract concentration. The effect of artichoke leaf extract on alcohol-induced HepG2 cell damage was evaluated by detecting the activity of intracellular ALT (alanine aminotransferase), AST (aspartate transaminase) and SOD (superoxide dismutase), the content of MDA (malondialdehyde) and TG (triglyceride) in each group of cell culture medium. Resules HepG2 cells were treated by 0.6%, 1.2%, and 2.4% alcohol concentration with 2.5, 5, and 10 μg/mL artichoke leaf extract for 48 h. Model group treated with the alcohol concentration, cell AST, ALT, TG, MDA levels were significantly increased and SOD activity decreased. With the increase of cell concentrations, the AST, ALT, TG and MDA level significantly decreased in treatment group comparing with the model group, SOD activity increased, and the protective effect on HepG2 cells increased. Conclusion Artichoke leaf extract can protect the alcohol impaired HepG2 cells to some extent, which has the potential of intensive research and development.