高通量测序技术研究高糖饮食对小鼠肠道菌群的影响

Comparision of intestinal microbiota in mice with normal and high-sugar diet using Miseq high-throughput sequencing

中文关键词:  高糖饮食  肠道菌群  高通量测序

英文关键词:high-sugar diet  microbiota  high-throughput sequencing

中文摘要:

      目的 比较高糖饮食和普通饲料喂养对小鼠肠道菌群的影响。方法 选取16只C57BL/6J小鼠, 经适应喂养1周后, 随机分为正常组和高糖组, 于第5周末, 对小鼠粪便菌群16S rRNA的V1-V3区进行扩增, Miseq高通量测序平台测定其基因序列, 与silva数据库中微生物基因序列进行比对、分类。结果 研究发现: 尽管差异性不显著, 高糖饮食组小鼠体重和血糖值较正常饮食小鼠有明显升高; 正常饮食小鼠粪便菌群多样性高于高糖饮食的小鼠; 高糖组粪便中含有大量的Firmicutes(厚壁菌门), 较正常组显著升高; 高糖组Bacteroidetes(拟杆菌门)数量显著低于正常组; 在属的水平上, 正常组和高糖组粪便菌群中含量最高的是Allobaculum, 其含量无显著差异; 而两组在Lactobacillus和S24-7_norank的含量上存在着显著差异。结论 饮食类型对肠道菌群有着显著的影响, 因而可通过调整饮食结构改善肠道菌群。

英文摘要:

      Objective To compare the composition of fecal microbiota in normal mice and high-sugar diet mice by using Miseq high-throughput sequencing technology. Methods Totally 16 C57BL/6J mice were randomly divided into 2 groups. The feces were collected after a 5-wk experimental period. The fecal bacterial composition was investigated by Miseq high-throughput sequencing of the V1-V3 region of the 16S rRNA gene compared with silva databases. Results The results showed that the body weight and blood glucose of mice with high-sugar diet were significantly increased compared to the mice fed normal diet. Bacterial diversity and richness in normal group was higher than that in high-sugar mice. In the phylum level, the relative abundance of Firmicutes was somewhat higher, while the proportion of Bacteroidetes was lower in high-sugar group compared with the normal group. In the genus level, Allobaculum was the most abundant bacterial in both groups, however, there was a significant difference in the percentage of Lactobacillus and S24-7_norank. Conclusion The diet has significant effect on gastro-intestinal microbiota, so we can improve the microbiota though regulating the structure of diet.