离子淌度差分质谱技术测定鸡肉中金刚烷胺和 利巴韦林

Differential mobility spectrometry for analysis of amantadine and ribavirin residues in chicken muscle

中文关键词:  离子淌度差分质谱  抗病毒药  鸡肉

英文关键词:differential mobility spectrometry  antiviral drugs  chicken muscle

中文摘要:

      目的 将离子淌度差分质谱(DMS)技术应用于鸡肉中金刚烷胺和利巴韦林抗病毒药物的残留量分析。方法 样品中的金刚烷胺和利巴韦林采用三氯乙酸提取, 经磷酸酯酶酶解, PBA固相萃取小柱净化后, 选用亲水相互作用色谱柱以5 mmol/L乙酸铵水溶液(含0.1%甲酸) -乙腈为流动相梯度洗脱, DMS结合ESI正离子模式定性定量分析。结果 在0.5~5.0 μg/L的浓度范围内线性关系良好, 方法定量限为1.0 μg/L, 标准添加水平在1.0、2.0、10 μg/L时, 回收率在68.4%~112.8%之间, 相对标准偏差(n=6)在7.3%~11.2%之间。结论 该技术的应用可以显著提高目标化合物的选择性, 有效去除基质中的干扰物质, 噪音明显降低。

英文摘要:

      Objective To establish a differential mobility spectrometry method for determination of amantadine and ribavirin residues in chicken muscle. Methods The amantadine and ribavirin in sample were extracted by trichloroacetic and enzyme hydrolyzing with acid phosphatase, then purified with PBA cartridge. The targeted compounds were separated on a HILIC column with the gradient elution of 5 mmol/L ammoni-umacetate (0.1% formic acid) and acetonitrile as the mobile phases. Quantitative and qualitative were detected by tandem mass spectrometry in positive electrospray ionization and multiple reaction monitoring (MRM) mode with differential mobility spectrometry (DMS) on. Results The method showed a good linearity in the range of 0.5~5.0 μg/L, and the limits of quantification was 1.0 μg/kg. The recoveries in chicken muscle at the 1.0 , 2.0 and 10 μg/kg spiked levels was in the range of 68.4%~112.8% with the relative standard deviations (RSD) in the range of 7.3%~11.2%. Conclusion This method can significantly improve the selection of target analysts, effectively eliminate the matrix interference and reduce the noise.