2011-15-3等4个茶树新品系亲缘关系的 EST-SSR分析

Analysis of genetic relationship of 2011-15-3 and other 3 new strains of tea based on EST-SSR markers

中文关键词:  新品系  亲缘关系  EST-SSR

英文关键词:new strain  genetic relationship  EST-SSR

中文摘要:

      Objective To analyze the genetic relationship of 2011-15-3 and other 3 new strains of tea, so as to offer some information for the new strains identification, new varieties registration and parental selection. Methods The 7 tea accessions were analyzed by the 24 pairs of EST SSR primers derived according to ref-erences. Based on EST-SSR data, a dendrogram was constructed by UPGMA method. Results 69 alleles were amplified totally using 24 EST-SSR primers, on average of 2.88. The observed heterozygosity (Ho), the expected heterozygosity (He) and the Shannon index were 0.42, 0.54 and 0.75 on average, respectively. The similarity coefficient varied from 0.47 to 0.89. The similarity coefficient offspring of Zhuyeqi, Bixiangzao and Gaoyaqi and their female parent were as high as 0.84, 0.79, 0.79 and 0.78, respectively. The 7 materials were classified into 3 groups based on the UPGMA method with the similarity coefficient at 0.66: the first group was Gaoyaqi and 2011-34-1, the second was Zhuyeqi, 2011-21-1 and 2011-21-3, and the third was Bixiangzao and 2011-15-3. Conclusion This paper estimated the phylogenetic relationship between 4 new strains of tea and other varieties and revealed the genetic distance among varieties.

英文摘要:

      Objective Genetic relationship of 2011-15-3 four new strains were analyzed so as to offer some information to be used in new strains identification, new varieties registration and parental selection. Method The 24 pairs of EST SSR primers were derived by the Liu Zhen and Zhao, were used to analyzed the 7 tea accessions. Based on EST-SSR data, a dendrogram was constructed using UPGMA method. Result It was showed that totally 69 alleles were amplified using 24 EST-SSR primers, on average of 2.88. The observed heterozygosity (Ho), the expected heterozygosity (He) and the Shannon index on average of 0.42, 0.54, 0.75 respectively. The similarity coefficient varied from 0.47 to 0.89, Zhuyeqi, Bixiangzao and Gaoyaqi of offspring and its female parent similarity coefficient as high as 0.84, 0.79, 0.79, 0.78 respectively. The 7 materials were classified into 3 groups based on the UPGMA method with the similarity coefficient at 0.66: the first group is Gaoyaqi and 2011-34-1, the second is Zhuyeqi , 2011-21-1, and 2011-21-3, the third is Bixiangzao and 2011-15-3. Conclusion We estimated the phylogenetic relationship between four new strains and other varieties and revealed genetic distance among varieties.