高效液相色谱法同时测定食品中7种防腐剂

Determination of 7 preservatives in food using high performance liquid chromatography

中文关键词:  高效液相色谱法  食品  防腐剂  苯甲酸  山梨酸  对羟基苯甲酸酯

英文关键词:high performance liquid chromatography  food  preservatives  benzoic acid  sorbic acid  p-hydroxybenzoic acid ester

中文摘要:

      目的 建立一种采用高效液相色谱同时检测食品中苯甲酸、山梨酸、脱氢乙酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯等7种防腐剂的方法, 并对葡萄酒、饮料、酱油、面包等4种基质进行测定。方法 样品经甲醇提取, 无水硫酸镁除水离心后取上清液经0.45 μm滤膜过滤, 使用高效液相色谱二极管阵列检测器(DAD)对7种防腐剂含量进行测定。结果 在优选条件下, 方法线性范围为0.1~10 mg/L, 线性相关系数R2 ≥0.9999, 检出限(S/N=3)为50~100 μg/L, 样品回收率为77.28%~106.81%, 相对标准偏差(RSD)为0.46%~2.38%。结论 该方法操作简便, 灵敏度高, 适用于葡萄酒、饮料、酱油和面包4种基质中7种防腐剂的检测。

英文摘要:

      Objective To establish a method for the simultaneous determination of 7 preservatives (benzoic acid, sorbic acid, dehydroacetic acid, methyl p-hydroxybenzoate, p-hydroxy benzoic acid ethyl ester, p-hydroxy benzoic acid n-propyl, and p-hydroxy benzoic acid butyl ester) in food by high performance liquid chromatography, and determine the wine, drink, soy sauce, and bread in the samples. Methods Samples were extracted with methanol, and assisted by MgSO4 to remove the water before the centrifugation. After through 0.45 μm filter, 7 kinds of preservatives were detected by HPLC-DAD. Results Under the optimized conditions, the linear range was 0.1~ 10 mg/L with R2≥0.9999. The limits of detection (LOD, S/N=3) were from 50 to 100 μg/L. Besides, recoveries were in the range of 77.28%~106.81%, and the RSDs of the peak areas were between 0.46%~2.38%. Conclusion This method is suitable for simple and sensitive determination of 7 kinds of preservatives in wine, drink, soy sauce and bread.