QuEChERS-酶联免疫快速检测法测定茶叶中 黄曲霉毒素B1

Simultaneous determination for aflatoxin B1 in tea leaf by QuEChERS -enzyme-linked immunosorbent assay

中文关键词:  QuEChERS  酶联免疫法  黄曲霉毒素B1  茶叶

英文关键词:QuEChERS  enzyme-linked immunosorbent assay  aflatoxin B1  tea leaf

中文摘要:

      目的 建立QuEChERS-酶联免疫快速检测茶叶中黄曲霉毒素B1的方法, 并对样品前处理条件进行优化。方法 茶叶样品用70%乙腈水提取溶液进行提取目标物, 离心后取上清液并用PSA+MgSO4进行净化处理后, 采用酶联免疫快速检测方法进行分析测定。结果 本方法的检出限为0.078 μg/kg, 线性范围为0.125~0.854 μg/kg, IC50值为0.327 ng/mL。在三个不同添加水平下, 样品的平均回收率为87.66%~97.17%, 相对标准偏差为4.89%~7.16%。检测结果与高效液相色谱法(high performance liquid chromatography, HPLC)方法的相关系r2=0.9854, 线性相关性良好。结论 该方法更加简便、快速、高效, 能够用于茶叶中黄曲霉毒素B1的检测。

英文摘要:

      Objective To establish a QuEChERS-enzyme-linked immunosorbent assay (ELISA) method for determining the aflatoxin B1 (AFB1) in tea leaf rapidly, and optimizing the sample pre-treatment conditions. Methods The samples were extracted with 70% acetonitrile in water, and the supernatant liquid was directly purified with PSA+MgSO4 for detection of ELISA. Results The limit of detection (LOD) of method was 0.078 μg/kg, with linear range between 0.125 and 0.854 μg/kg, and IC50 value was 0.327 ng/mL. Average recovery rate of samples was between 87.66% and 97.17% at 3 adding levels, and the relative standard deviation (RSD) was 4.89%~7.16%. The results showed a good coefficient with high performance liquid chromatography (HPLC) (r2=0.9854). Conclusion This method proved to be suitable for the screening of tea leaf samples for the presence of AFB1, which was easier, faster and more efficient.