酸消化-气相色谱法测定鱼肉中指示性 多氯联苯研究

Determination of indicator polychlorinated biphenyls residues in the flesh of fish by gas chromatography using acid digestion

中文关键词:  鱼肉  多氯联苯  酸消化  气相色谱法

英文关键词:flesh of fish  polychlorinated biphenyls  acid digestion  gas chromatography

中文摘要:

      目的 建立酸消化-气相色谱法测定鱼肉中指示性多氯联苯的方法。方法 用硫酸溶液消化样品, 以正己烷+二氯甲烷(50:50, v:v)为提取溶液萃取、浓硫酸净化后用毛细管气相色谱法测定。结果 采用该方法对鳕鱼中多氯联苯标准物质GBW(E)100131进行检测, 检测结果与参考物定值吻合, 回收率为93.7%~104.4%, 相对标准偏差为2.75%~4.65%, 检出限0.22~0.44 μg/kg。结论 该方法具有简单、快速、测定灵敏度高和精确度高的特点, 能满足鱼肉中指示性多氯联苯分析的要求, 值得推广应用。

英文摘要:

      Objective To establish a method to detect the residual indicator polychlorinated biphenyls (PCBs) in the flesh of fish by gas chromatography using acid digestion. Methods The sample was digested by H2SO4, then extracted by N-hexane and methylene chloride (50:50, v:v) and cleaned up by H2SO4 , after that determined by capillary gas chromatography. Results PCBs reference material GBW(E)100131 in Cod was detected by this method and the result was in accordance with the assigned value. The recovery was 93.7%~104.4%, RSD was 2.75%~4.65% (n=6), and the detection limit was 0.22~0.44 μg/kg. Conclusion The method is simple, rapid, sensitive, accurate and precise, and it can be used for the determination of indicator polychlorinated biphenyls in the flesh of fish.