离子色谱-电导检测法快速测定乳与乳制品中 硫氰酸根含量的研究

Study on determination of thiocyanate ion in milk and dairy products by ion chromatography-conductivity detection

中文关键词:  离子色谱-电导检测法  硫氰酸根  测定

英文关键词:ion chromatography-conductivity test  thiocyanate ion  determination

中文摘要:

      目的 建立一种离子色谱-电导检测法快速测定乳与乳制品中硫氰酸根含量的方法。方法 样品经水和甲醇震荡提取、离心后上清液用滤膜过滤后, AS16分离柱分离, 由35 mmol/L氢氧化钾溶液等度洗脱, 用电导检测器检测, 外标法定量。结果 方法的线性范围为0.02~20 μg/mL, R=0.9990, 检出限为0.1 mg/kg, 相对标准偏差为0.89%~3.31%, 回收率范围为96%~105%。结论 该离子色谱法快速、准确、精密度高, 适用于乳与乳制品中硫氰酸根的批量检测。

英文摘要:

      Objective To establish a rapid method for the determination of thiocyanate ion in milk and dairy products by ion chromatography (IC) method . Methods The samples were extracted by water and methanol, then centrifuged, supernatant fluid were filtered by filter membrane. The samples were analyzed using AS16 column, and elution with the mobile phase of 35 mmol/L potassium hydroxide, and detected by conduc-tivity detector, the external standard was used for quantitative. Results The linearity of the method was 0.02~20 μg/mL (R=0.9990), the limit of detection was 0.1 mg/kg, and the relative standard deviations were 0.89%~3.31%, the average recoveries were 96%~105%. Conclusion This method is a rapid, accurate, and of high precision method, and is suitable for the requirements of the thiocyanate ion analysis.