低温离心净化-高效液相色谱法快速测定 腐竹中的碱性橙II和碱性嫩黄O

Rapid determination of chrysoidine II and auramine O in dried bean curd sticks by cryogenic centrifugal purification–high performance liquid chromatography

中文关键词:  低温离心净化  高效液相色谱  二极管阵列检测器  腐竹  碱性橙II  碱性嫩黄O

英文关键词:cryogenic centrifugal purification  high performance liquid chromatography  photodiode array detector  dried bean curd sticks  chrysoidine II  auramine O

中文摘要:

      目的 建立腐竹中碱性橙II和碱性嫩黄O的低温离心净化-高效液相色谱测定方法。方法 样品经70%的乙腈-氨水(0.5%)提取，4 ℃低温、12000 r/min离心净化，高效液相色谱(high performance liquid chromatography, HPLC)分离, 最后采用二极管阵列检测器(PDA)检测, 以保留时间(Rt)定性, 外标法定量。结果 在0.00125~0.05 mg/mL的浓度范围内, 标准曲线有良好的线性关系, 相关系数大于0.9999; 样品在0.05、2.0、10.0 mg/kg三个加标水平内的回收率范围为95.0%~103.0%(n=10), 相对标准偏差小于5.0%。方法的检出限(LOD)为0.02 mg/kg, 定量限(LOQ)为0.05 mg/kg。结论 对实际样品测试结果与参考文献方法比较, 该方法具有简便、快速、灵敏、费用低, 便于推广应用。

英文摘要:

      Objective To establish a method to detect chrysoidine II and auramine O in dried bean curd sticks by cryogenic centrifugal purification–high performance liquid chromatography. Methods The samples were diluted and extracted by 70% acetonitrile ammonia water, purified by centrifugation at 12000 r/min and 4 ℃, separated by high performance liquid chromatography (HPLC), and detected by photodiode array de-tector (PDA). The method took the retention time (Rt) for qualitative and external standard method quantitative analysis. Results Within the limits of concentration 0.00125~0.05 mg/mL, the standard curve had a good linear relationship, and the correlation coefficient was more than 0.9999; in the level of 0.05, 2.0, and 10.0 mg/kg, the add mark recovery rate was from 95.0% to 103.0% (n=10), the relative standard deviation was less than 5.0%; limit of detection(LOD) was 0.02 mg/kg, limit of quantitation (LOQ) was 0.05 mg/kg. Conclusion Comparing the actual sample testing results with reference method, this method is simple, rapid, sensitive, with low cost, and is suitable for popularization and application.