10种蜂蜜中的抗氧化物质及其抗氧化能力分析

Analysis of antioxidant substance derived from 10 kinds of honey and their antioxidant activity

中文关键词:  蜂蜜  抗氧化物质  抗氧化能力  相关性分析

英文关键词:honey  antioxidant substance  antioxidant capacity  correlation analysis

中文摘要:

      目的 分析市售蜂蜜与农家蜂蜜中抗氧化物质含量的多少, 为我国蜂蜜品质的评价和鉴别提供一定的基础。方法 采用分光光度计法测定3种市售蜂蜜和7种农家蜂蜜中总酚、总黄酮、脯氨酸含量、DPPH自由基清除能力、超氧阴离子清除能力及总抗氧化能力, 通过数据间的显著性分析和相关性分析明确各指标之间的关系。结果 市售蜂蜜和农家蜂蜜总酚含量均值分别为24.93 mg/100 g和30.32 mg/100 g; 市售3种蜂蜜中的总黄酮含量均低于20 mg/100 g, 而农家的7种蜂蜜均高于20 mg/100 g; 农家蜂蜜脯氨酸含量的均值是市售蜂蜜的2.5倍之多。市售蜂蜜的DPPH清除能力、超氧阴离子清除作用和总抗氧化能力均高于农家蜂蜜。蜂蜜的抗氧化能力与总酚、总黄酮和脯氨酸含量呈显著的正相关。结论 10种蜂蜜的各项指标间差异显著, 农家蜂蜜的抗氧化物质含量高于市售蜂蜜, 而市售蜂蜜的抗氧化能力高于农家蜂蜜, 蜂蜜的抗氧化物质与抗氧化能力间呈显著的相关性。

英文摘要:

      Objective To analyze the content of antioxidant substance in commercially and farmhouse available honey, which was helpful to the evaluation and identification of honey. Method Total phenols, total flavonoids, proline, DPPH free radical scavenging capacity, super oxygen anion removal ability and total antioxidant capacity of 3 kinds of market available honey and 7 kinds of farmhouse available honey were analyzed by spectrophotometer. The relationship of different samples was analyzed by significance analysis and correlation analysis. Results The content of total phenols in commercially available and farmhouse available honey was 24.93 mg/100 g and 30.32 mg/100 g, respectively. The content of total flavonoids in commercially available honey was lower than 20 mg/100 g, while in farmhouse available honey was higher than 20 mg/100 g. The content of proline in farmhouse honey was 2.5 times higher than that of commercially available honey. The DPPH free radical scavenging capacity, super oxygen anion removal ability and total antioxidant capacity in commercially available honey were higher than those of farmhouse honey. Total antioxidant capacity of honey had positive relationship with total phenols, total flavonoids and proline. Conclusions There is a significant difference in the properties. The content of antioxidant substance in farmhouse honey are higher than that of commercially available honey, but the antioxidant activity has the opposite results. The relationship between the content of antioxidant substance and antioxidant activity is of significant correlation.