荧光法在食品理化检验标准中的应用进展

Application of fluorescence detection in food inspection standards

中文关键词:  荧光  食品安全标准  检验

英文关键词:fluorescence  food standards  detection

中文摘要:

      近年来, 荧光检测法以其灵敏度高、特异性强、易于操作及定量精准等优点, 在食品理化检验领域获得了越来越多的应用, 其相应的检验标准, 包括国家标准、农业部标准、检验检疫行业标准、农业部公告、地方标准等也日渐增多, 其更新修订的频率与数量也逐年递增。本文对我国现行有效的理化标准中所采用的荧光检测方法, 包括荧光分光光度法、薄层色谱-荧光法、高效液相色谱-荧光法、原子荧光法等进行了总结, 并着重对液相色谱荧光法中的目标物提取、免疫亲和净化、分离检测条件等进行了论述, 最后对荧光检测的当前热点以及未来发展趋势进行了展望。本文为相关科研人员在荧光检测领域的标准起草、草案建议、方法开发及日常食品安全分析检验提供了有力的技术支持。

英文摘要:

      In recent years, fluorescence detection has aroused more and more interest due to its high sensitivity, high selectivity, user-friendly and accurate quantification. Accordingly, the number of the standards, including the GB standards, the NY standards, the SN standards, the Ministry of Agriculture Announcements and the DB standards were increasing every year, the revisions and the adjustments also became frequent. The effective food safety standards in China using fluorescence detection, including the fluorescence spectrophotometry, high-performance liquid chromatography with fluorescence detector, thin-layer chromatography with fluorescence detector and atomic fluorescence spectrometry were summarized in this review. The high-performance liquid chromatography with fluorescence detector method, including the extraction of the target compound, immunoaffinity column cleanup, the excitation wavelength and emission wavelength of the fluorescence were discussed in detail. In the end, we also discussed the existing problem and the future trends in this field. This article can provide useful technique support to the relevant researchers in the fields of formulating the standards, supporting the methods, developing the new methods and regular food analytical detection using fluorescence detection.