MALDI-TOF MS方法快速鉴定大肠杆菌及ESBLs菌株检测探索研究

Exploration and research of rapidly identifying escherichia coli and detecting ESBLs strains by matrix-assisted laser desorption ionization-time of flight mass spectrometry

中文关键词:  大肠杆菌  超广谱β-内酰胺酶  检测  基质辅助激光解析电离飞行时间质谱法

英文关键词:Escherichia coli  extended spectrum β-Lactamases  detection  matrix-assisted laser desorption ionization-time of flight mass spectrometry

中文摘要:

      目的 研以基质辅助激光解析电离飞行时间质谱(matrix assisted laser desorption ionization-time of flight mass spectrometry, MALDI-TOF MS)方法检测大肠杆菌, 并利用MALDI-TOF MS方法鉴别ESBLs菌株及聚类分析法区分不同分型的ESBLs菌株, 探索MALDI-TOF MS方法鉴定大肠杆菌及ESBLs菌株的可行性。方法 利用MALDI-TOF MS采集47株大肠杆菌分离株的质谱数据, 生成肽指纹图谱, 并利用biotyper软件进行菌种鉴定, 同时研究其耐药性鉴定结果, 并利用聚类分析法研究大肠杆菌ESBLs菌株, 最终确定MALDI-TOF MS对大肠杆菌菌种鉴定及ESBLs菌株鉴别的可行性。结果 MALDI-TOF MS方法鉴定大肠杆菌的结果与传统方法及PCR方法检测结果均符合, 经MALDI-TOF MS鉴定方法提示为ESBLs菌株的10株大肠杆菌中有9株为ESBLs菌株, 且聚类分析法对OXA型ESBLs大肠杆菌具有较好的鉴别能力。结论 MALDI-TOF MS方法可以快速、准确地鉴定大肠杆菌, 对ESBLs菌株具有一定的筛选鉴别能力。

英文摘要:

      Objective The strains of Escherichia coli was tested by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). ESBLs strains were distinguished, and different type ESBLs strains was differentiated by clustering analysis. Feasibility of identification was explored by MALDI-TOF MS for E.coli and ESBLs strains. Methods Peptide mass fingerprints were obtained via collecting mass spectrometric data of 47 E.coli. Their species were identified by biotyper sortware, and drug resistance results were studied. ESBLs strains of E.coli were researched by clustering analysis. Feasibility of identification was confirmed by MALDI-TOF MS for E.coli and ESBLs strains. Results The identification results were coincident with conventional method and PCR method. There were 9 ESBLs stains in 10 E.coli that were prompted for ESBLs strains by MALDI-TOF MS. It had good distinguishing capability for OXA type ESBLs E.coli by cluster analysis. Conclusion E.coli strains could be rapidly, accurately identified by MALDI-TOF MS, and there was a certain screening capacity for ESBLs strains.