基于生物量的杏鲍菇菌株筛选及液态摇瓶发酵 培养条件的优化

Screening of strains and optimization of culture conditions with shaking flask fermentation of Pleurotus eryngii based on the biomass

中文关键词:  杏鲍菇  菌株筛选  深层发酵  正交实验  响应面分析

英文关键词:Pleurotus eryngii  strain screening  submerged fermentation  orthogonal test  response surface methodology

中文摘要:

      目的 筛选得到适于液态摇瓶发酵的杏鲍菇菌株, 并优化其最佳培养条件。方法 采用单因素方法筛选发酵最佳培养基成分及发酵条件、采用正交试验优化最佳培养基配方、利用Box-Behnken中心组合试验, 对发酵过程中影响杏鲍菇菌丝生长的三个关键因素(温度、转速和发酵周期)进行优化, 最后用响应面分析方法对试验数据进行分析。结果 优化后的发酵培养基组分为: 葡萄糖3.50 g/100 mL, 胰蛋白胨0.50 g/100 mL, 磷酸二氢钾0.30 g/100 mL, 硫酸镁0.20 g/100 mL, pH自然。当发酵温度为25 ℃, 摇床转速170 r/min, 发酵周期7.5 d(180 h)时, 杏鲍菇菌丝体生物量(干重)达1.687 g/100 mL。结论 采用单因素、正交、响应面等试验方法筛选得到了菌株的最佳摇瓶发酵培养条件。

英文摘要:

      Objective To screen Pleurotus eryngii strain suitable for shaking flask fermentation, and the best culture conditions was optimized. Methods The fermentation culture medium and fermentation conditions were optimized by the single factor method, the optimum medium formula was obtained by orthogonal experiment, the three key factors (temperature, speed and fermentation cycle) were optimized by Box-Behnken design principles, and the experimental data were analyzed by response surface analysis. Results The result showed that glucose 3.50 g/100 mL, tryptone 0.50 g/100 mL, monopotassium 0.30 g/100 mL, magnesium sulfate 0.20 g/100 mL were the best medium, pH naturally. Under the conditions of 25 ℃, 170 r/min and 7.5 d (180 h), the mycelium biomass reached 1.687 g/100 mL. Conclusion The optimal fermentation culture conditions were screened by single factor, orthogonal and response surface experimental methods.