高效液相色谱-紫外检测法测定水产品中组胺含量

Determination of histamine content in aquatic products by high performance liquid chromatography with ultraviolet detector

中文关键词:  水产品  组胺含量  高效液相色谱法  紫外检测法  甲醇

英文关键词:aquatic products  the content of histamine  high performance liquid chromatography  ultraviolet detector  methanol

中文摘要:

      目的 对高效液相色谱法测定水产品(鲭鱼)中组胺含量的试验方法进行改进, 建立一种提取方法简单、衍生物稳定、操作方便的检测方法。方法 用0.4 mol/L高氯酸溶液提取水产品中的组胺, 采用10 mg/mL丹磺酰氯溶液作为衍生剂进行衍生, 用甲醇、水的流动相梯度洗脱, 高效液相色谱-紫外检测法测定水产品中的组胺含量。结果 该方法提取完全、操作简单、峰型理想、组胺出峰干扰少, 回收率大于90%, 方法检出限为50 mg/kg。结论 该方法能够满足水产品中组胺含量的测定要求, 是一种理想的组胺检测方法。

英文摘要:

      Objective To improve the method of high performance liquid chromatography (HPLC) for determination of histamine in aquatic product (mackerel fish), and to develop a method with simple extraction, stable derivatives, and easy to operate. Method The perchloric acid solution 0.4 mol/L was used to extract histamine of aquatic products, dansyl chloride solution 10 mg/mL as derivative agent, methanol and water as the HPLC flow phase to gradient elution. The histamine content was detected by high performance liquid chromatography with ultraviolet detector. Results The method had the advantages of complete extraction, simple operation, ideal peak, less interference of histamine peak. The recovery rate was more than 90%, and the detection limit was 50 mg/kg. Conclusion It can meet the requirements of determination of histamine in aquatic products, and is an ideal method for detection of histamine.