入境马蹄莲细菌性软腐病菌的分离鉴定

Isolation and identification of soft rot bacteria on imported Zantedeschia

中文关键词:  马蹄莲  细菌性软腐病  病原鉴定  边缘假单胞

英文关键词:Zantedeschia  soft rot bacteria  pathogen identification  Pseudomonas marginalis

中文摘要:

      目的 在参展青岛世界园艺博览会的荷兰进口马蹄莲植株上, 发现叶片有明显褐色软腐症状, 为明确引起马蹄莲软腐病的病原, 对叶片进行病菌的分离鉴定。方法 采用组织分离法, 从荷兰入境马蹄莲植株的病变叶片中分离纯化到2株致病性细菌菌株(MTL1、MTL2), 对该菌株进行鉴定和生物学特性研究。结果 形态及培养特征、生理生化特性的研究结果表明该菌株在NA培养基上能形成白色圆形单菌落, 薄而平滑、边缘整齐, 光滑不透明, KB培养基上划线培养后可产生明显的绿色的荧光。显微镜下菌体呈杆状, 具多根极生鞭毛, 革兰氏阴性菌。结论 经形态鉴定、培养特征、生理生化及16S rDNA序列分析和致病性测定实验, 确认引起马蹄莲细菌性软腐病的病原为边缘假单胞菌(Pseudomonas marginalis)。

英文摘要:

      Objective To know the pathogens of brown soft rot bacteria of Zantedeschia imported from Holland. Methods Two pathogenic bacterial strains were isolated from imported Zantedeschia leaves by tissue isolation method. The strains were identified and their biological characteristics were studied. Results Morphological characteristics, culture patterns and physiological and biochemical reactions results indicated that all strains formed white, glossy, opaque, thin and smooth, round single colony on nutrient agar (NA) while produced green fluorescence in the KB medium. The bacterial cells were gram-negative rods with several polar flagellum under the microscope. Conclusion According to the pathogenicity, staining reactions, morphological characteristics, culture patterns, physiological and biochemical reactions, and 16S rDNA gene sequences analysis results, the isolated strain was identified as Pseudomonas marginalis.